Abstract | OBJECTIVE: METHODS: RESULTS: Among the test compounds, umbelliferone 6-carboxylic acid, esculetin and daphnetin exhibited potent inhibitory activity against AChE, BChE and BACE1. Both esculetin and daphnetin have a catechol group and exhibit significant anti-AD activity against AChE and BChE. In contrast, presence of a sugar moiety and methoxylation markedly reduced the anti-AD activity of the coumarins investigated in this study. With respect to BACE1 inhibition, umbelliferone 6-carboxylic acid, esculetin and daphnetin contained carboxyl or catechol groups, which significantly contributed to their anti-AD activities. To further investigate these results, we generated a 3D structure of BACE1 using Autodock 4.2 and simulated binding of umbelliferone 6-carboxylic acid, esculetin and daphnetin. Docking simulations showed that different residues of BACE1 interacted with hydroxyl and carboxylic groups, and the binding energies of umbelliferone 6-carboxylic acid, esculetin and daphnetin were negative (-4.58, -6.25 and -6.37 kcal/mol respectively). CONCLUSIONS:
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Authors | Md Yousof Ali, Susoma Jannat, Hyun Ah Jung, Ran Joo Choi, Anupom Roy, Jae Sue Choi |
Journal | Asian Pacific journal of tropical medicine
(Asian Pac J Trop Med)
Vol. 9
Issue 2
Pg. 103-11
(Feb 2016)
ISSN: 2352-4146 [Electronic] India |
PMID | 26919937
(Publication Type: Journal Article)
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