Biodentine Reduces Tumor Necrosis Factor Alpha-induced TRPA1 Expression in Odontoblastlike Cells.

Abstract

INTRODUCTION:

The transient receptor potential (TRP) ion channels have emerged as important cellular sensors in both neuronal and non-neuronal cells, with TRPA1 playing a central role in nociception and neurogenic inflammation. The functionality of TRP channels has been shown to be modulated by inflammatory cytokines. The aim of this study was to investigate the effect of inflammation on odontoblast TRPA1 expression and to determine the effect of Biodentine (Septodent, Paris, France) on inflammatory-induced TRPA1 expression.

METHODS:

Immunohistochemistry was used to study TRPA1 expression in pulp tissue from healthy and carious human teeth. Pulp cells were differentiated to odontoblastlike cells in the presence of 2 mmol/L beta-glycerophosphate, and these cells were used in quantitative polymerase chain reaction, Western blotting, calcium imaging, and patch clamp studies.

RESULTS:

Immunofluorescent staining revealed TRPA1 expression in odontoblast cell bodies and odontoblast processes, which was more intense in carious versus healthy teeth. TRPA1 gene expression was induced in cultured odontoblastlike cells by tumor necrosis factor alpha, and this expression was significantly reduced in the presence of Biodentine. The functionality of the TRPA1 channel was shown by calcium microfluorimetry and patch clamp recording, and our results showed a significant reduction in tumor necrosis factor alpha-induced TRPA1 responses after Biodentine treatment.

CONCLUSIONS:

In conclusion, this study showed TRPA1 to be modulated by caries-induced inflammation and that Biodentine reduced TRPA1 expression and functional responses.

• Authors: Ikhlas A El Karim, Maelíosa T C McCrudden, Mary K McGahon, Tim M Curtis, Charlotte Jeanneau, Thomas Giraud, Chris R Irwin, Gerard J Linden, Fionnuala T Lundy, Imad About
• Journal: Journal of endodontics (J Endod) Vol. 42 Issue 4 Pg. 589-95 (Apr 2016) ISSN: 1878-3554 [Electronic] United States
• PMID: 26874643 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

Chemical References

• Calcium Channels
• Calcium Compounds
• Glycerophosphates
• Nerve Tissue Proteins
• Silicates
• TRPA1 Cation Channel
• TRPA1 protein, human
• TRPV Cation Channels
• Transient Receptor Potential Channels
• Tumor Necrosis Factor-alpha
• tricalcium silicate
• beta-glycerophosphoric acid

Topics

• Calcium Channels (biosynthesis, genetics)
• Calcium Compounds (pharmacology)
• Cell Differentiation (drug effects)
• Dental Caries (metabolism)
• Dental Pulp (drug effects, pathology)
• Dental Pulp Capping
• Glycerophosphates (pharmacology)
• Humans
• Immunohistochemistry
• Nerve Tissue Proteins (biosynthesis, genetics)
• Odontoblasts (drug effects, metabolism, pathology)
• Silicates (pharmacology)
• TRPA1 Cation Channel
• TRPV Cation Channels (drug effects, metabolism)
• Transient Receptor Potential Channels (biosynthesis, genetics)
• Tumor Necrosis Factor-alpha (pharmacology)