Abstract | BACKGROUND: METHODS: Three types of tissue samples (primary CRC lesions without liver metastasis, primary lesions with liver metastasis, and liver metastatic tissues) were used for miRNA profiling to identify differentially expressed miRNA. Quantitative real-time PCR was used to examine miRNA expression in CRC cells and in tumor tissues. RESULTS: MiR-132 was significantly down-regulated in primary CRC tissues with liver metastasis and liver metastatic lesions compared to primary lesions without liver metastasis. Multivariate analysis for overall survival indicated that low miR-132 expression was an independent prognostic factor for CRC patients (overall survival P = 0.040, disease-free survival P = 0.015). Ectopic expression of miR-132 significantly inhibited cell proliferation and cell invasion. The luciferase reporter assay revealed that anoctamin 1 (ANO1) was a direct target of miR-132. Kaplan-Meier survival curves showed that high ANO1 expression was a significant prognostic factor for overall survival of patients with CRC (P = 0.0344). CONCLUSIONS: Down-regulation of miR-132 is associated with poor prognosis in CRC. ANO1 could be one of the crucial targets of miR-132 in CRC.
|
Authors | Yukako Mokutani, Mamoru Uemura, Koji Munakata, Daisuke Okuzaki, Naotsugu Haraguchi, Hidekazu Takahashi, Junichi Nishimura, Taishi Hata, Kohei Murata, Ichiro Takemasa, Tsunekazu Mizushima, Yuichiro Doki, Masaki Mori, Hirofumi Yamamoto |
Journal | Annals of surgical oncology
(Ann Surg Oncol)
Vol. 23
Issue Suppl 5
Pg. 599-608
(12 2016)
ISSN: 1534-4681 [Electronic] United States |
PMID | 26868958
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- 3' Untranslated Regions
- Anoctamin-1
- MIRN132 microRNA, human
- MicroRNAs
|
Topics |
- 3' Untranslated Regions
- Anoctamin-1
(genetics, metabolism)
- Cell Movement
(genetics)
- Cell Proliferation
(genetics)
- Colorectal Neoplasms
(genetics, pathology)
- Disease-Free Survival
- Down-Regulation
- Female
- HCT116 Cells
- Humans
- Liver Neoplasms
(genetics, secondary)
- Male
- MicroRNAs
(genetics)
- Oligonucleotide Array Sequence Analysis
- Survival Rate
- Transfection
|