Association of Ulcerative Colitis with FUT2 and FUT3 Polymorphisms in Patients from Southeast China.

Abstract

OBJECTIVES:

Dysbiosis of intestinal microbiota has been implicated in ulcerative colitis (UC). Fucosyltransferase (FUT) 2 and FUT3 determine expression of histo-blood group antigens in the gut and may affect the intestinal microbiota. We investigated the association between FUT2 and FUT3 polymorphisms and UC in Chinese patients.

METHODS:

We genotyped FUT2 (rs281377, rs1047781 and rs601338) and FUT3 (rs28362459, rs3745635 and rs3894326) in 485 UC patients and 580 healthy controls using SNaPshot. We also evaluated expression of Lewis a and b antigens in the sigmoid colon of 7 UC patients and 7 patients with benign colonic polyps.

RESULTS:

The frequencies of mutant allele (A) and genotype (GA+AA) in FUT3 (rs3745635) were higher in UC patients than controls (P = 0.016, 95%CI: 1.339-1.699; P = 0.038, 95%CI: 1.330-1.742, respectively). Stratified analyses revealed that the frequencies of mutant allele (G) and genotype (TG+GG) of FUT3 (rs28362459) were significantly lower in patients with extensive colitis than those with distal colitis (P<0.001, 95%CI: 0.503-0.742; P = 0.001, 95%CI: 0.567-0.786, respectively). Similar conclusions were drawn for the mutant allele (A) and genotype (GA+AA) of FUT3 (rs3745635) in patients with extensive colitis compared to those with distal colitis (P = 0.006, 95%CI: 0.553-0.845; P = 0.011, 95%CI: 0.621-0.900, respectively). Although expression of Lewis b antigen in the sigmoid colon did not differ between UC patients and controls, Lewis a antigen expression was higher in the cryptic epithelium of both inflammatory and non-inflammatory sigmoid colon of UC patients than controls (P = 0.028).

CONCLUSIONS:

Our findings indicated that polymorphisms in FUT3 and its intestinal expression might be associated with UC pathogenesis.

• Authors: Dingyuan Hu, Daguan Zhang, Shuzi Zheng, Maodong Guo, Xinxin Lin, Yi Jiang
• Journal: PloS one (PLoS One) Vol. 11 Issue 1 Pg. e0146557 ( 2016) ISSN: 1932-6203 [Electronic] United States
• PMID: 26766790 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Fucosyltransferases
• 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase
• galactoside 2-alpha-L-fucosyltransferase

Topics

• Adult
• Case-Control Studies
• China
• Colitis, Ulcerative (genetics)
• Female
• Fucosyltransferases (genetics, metabolism)
• Humans
• Intestinal Mucosa (metabolism)
• Male
• Middle Aged
• Polymorphism, Single Nucleotide