Abstract |
The aim of this research was to study polymorphisms in the genes encoding cytokines interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) in patients with celiac disease ( CD) antigens DQ2 (DQ2-positive) or DQ8 (DQ8-positive). We compared the results with healthy controls to determine whether any of the polymorphisms have a role in susceptibility to CD. A case-control of 192 patients with CD (96 DQ2-positive and 96 DQ8-positive) and 96 healthy controls from northeast Italy were included in the study. Analysis of single nucleotide polymorphisms (SNPs) was carried out using the polymerase chain reaction-restriction fragment length polymorphism method. Significant differences for the TNF-α(-308 G>A) polymorphism were observed when we compared the flowing groups: DQ2-positive with controls [odds ratio (OR) = 0.45, P = 0.0002]; DQ8-positive with controls (OR = 3.55, P < 0.0001); and DQ2-positive with DQ8-positive (OR = 0.12, P < 0.0001). We did not observe a statistically significant association between IL-6 (-174 G>C) polymorphism and CD (P > 0.05). Our results suggest that TNF-α(-308 G>A) polymorphism may play a role in susceptibility to CD in Italian patients.
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Authors | R M de Albuquerque Maranhão, F A Martins Esteves, S Crovella, L Segat, P R Eleutério Souza |
Journal | Genetics and molecular research : GMR
(Genet Mol Res)
Vol. 14
Issue 4
Pg. 16343-52
(Dec 09 2015)
ISSN: 1676-5680 [Electronic] Brazil |
PMID | 26662429
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- HLA-DQ Antigens
- Interleukin-6
- Tumor Necrosis Factor-alpha
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Topics |
- Adolescent
- Adult
- Aged
- Alleles
- Case-Control Studies
- Celiac Disease
(epidemiology, genetics)
- Child
- Child, Preschool
- Female
- Gene Frequency
- Genetic Association Studies
- Genetic Predisposition to Disease
- Genotype
- HLA-DQ Antigens
(genetics)
- Humans
- Interleukin-6
(genetics)
- Italy
(epidemiology)
- Male
- Middle Aged
- Polymorphism, Single Nucleotide
- Tumor Necrosis Factor-alpha
(genetics)
- Young Adult
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