Abstract |
Transforming growth factor-β/Smad3 signaling plays a critical role in the process of chronic kidney disease (CKD), but targeting Smad3 systematically may cause autoimmune disease by impairing immunity. In this study, we used whole-transcriptome RNA-sequencing to identify the differential gene expression profile, gene ontology, pathways, and alternative splicing related to TGF-β/Smad3 in CKD. To explore common dysregulation of genes associated with Smad3-dependent renal injury, kidney tissues of Smad3 wild-type and knockout mice with immune (anti-glomerular basement membrane glomerulonephritis) and non-immune (obstructive nephropathy)-mediated CKD were used for RNA-sequencing analysis. Totally 1922 differentially expressed genes (DEGs) were commonly found in these CKD models. The up-regulated genes are inflammatory and immune response associated, while decreased genes are material or electron transportation and metabolism related. Only 9 common DEGs were found to be Smad3-dependent in two models, including 6 immunoglobulin genes (Ighg1, Ighg2c, Igkv12-41, Ighv14-3, Ighv5-6 and Ighg2b) and 3 metabolic genes (Ugt2b37, Slc22a19, and Mfsd2a). Our results identify transcriptomes associated with renal injury may represent a common mechanism for the pathogenesis of CKD and reveal novel Smad3 associated transcriptomes in the development of CKD.
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Authors | Qin Zhou, Yuanyan Xiong, Xiao R Huang, Patrick Tang, Xueqing Yu, Hui Y Lan |
Journal | Scientific reports
(Sci Rep)
Vol. 5
Pg. 17901
(Dec 09 2015)
ISSN: 2045-2322 [Electronic] England |
PMID | 26648110
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- RNA, Messenger
- Smad3 Protein
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Topics |
- Animals
- Computational Biology
- Disease Models, Animal
- Gene Expression Profiling
- Gene Expression Regulation
- Gene Ontology
- Genome
- Genomics
- High-Throughput Nucleotide Sequencing
- Mice
- Mice, Knockout
- RNA Splicing
- RNA, Messenger
(genetics)
- Renal Insufficiency, Chronic
(genetics, metabolism, pathology)
- Reproducibility of Results
- Signal Transduction
- Smad3 Protein
(genetics, metabolism)
- Transcriptome
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