Cytotoxic Clerodane Diterpenoids from the Leaves of Casearia grewiifolia.

Abstract

Two new clerodane diterpenoids (1 and 2) and the known compound caseanigrescen D (3) were isolated from the leaves of Casearia grewiifolia by bioassay-guided fractionation. Their structures were determined by analyses of MS and 2D NMR data. The absolute configurations of 1 and 3 were established by analysis of X-ray diffraction data. Compounds 1-3 were evaluated for their cytotoxicity against four cancer cell lines, KB (mouth epidermal carcinoma cells), HepG-2 (human liver hepatocellular carcinoma cells), LU-1 (human lung adenocarcinoma cells), and MCF-7 (human breast cancer cells). Caseagrewifolin B (2) had inhibitory activity against KB and HepG-2 cell lines with IC50 values of 6.2 to 7.0 μM, respectively. When tested against the normal cells (NIH/3T3), caseagrewifolin B (2) exhibited a significant selective inhibition against cancer cells in comparison with the normal cells. Caseanigrescen D (3) was cytotoxic against four cancer cell lines; however it had no selective inhibition compared with normal cells.

• Authors: Ha T T Nguyen, Ngan B Truong, Huong T M Doan, Marc Litaudon, Pascal Retailleau, Thao T Do, Hung V Nguyen, Minh V Chau, Cuong V Pham
• Journal: Journal of natural products (J Nat Prod) Vol. 78 Issue 11 Pg. 2726-30 (Nov 25 2015) ISSN: 1520-6025 [Electronic] United States
• PMID: 26516994 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents, Phytogenic
• Diterpenes, Clerodane
• caseagrewifolin B
• caseagrewiifolin A

Topics

• Antineoplastic Agents, Phytogenic (chemistry, isolation & purification, pharmacology)
• Casearia (chemistry)
• Diterpenes, Clerodane (chemistry, isolation & purification, pharmacology)
• Drug Screening Assays, Antitumor
• Female
• Hep G2 Cells
• Humans
• KB Cells
• Molecular Structure
• Plant Leaves (chemistry)
• Plants, Medicinal (chemistry)
• Vietnam