Randomized in vivo evaluation of photodynamic antimicrobial chemotherapy on deciduous carious dentin.

Abstract

The aim of this randomized in vivo study was to compare antimicrobial chemotherapies in primary carious dentin. Thirty-two participants ages 5 to 7 years underwent partial caries removal from deep carious dentin lesions in primary molars and were subsequently divided into three groups: control [chlorhexidine and resin-modified glass ionomer cement (RMGIC)], LEDTB [photodynamic antimicrobial chemotherapy (PACT) with light-emitting diode associated with toluidine blue solution and RMGIC], and LMB [PACT with laser associated with methylene blue solution and RMGIC]. The participants were submitted to initial clinical and radiographic examinations. Demographic features and biofilm, gingival, and DMFT/DMFS indexes were evaluated, in addition to clinical and radiographic followups at 6 and 12 months after treatments. Carious dentin was collected before and after each treatment, and the number of Streptococcus mutans, Streptococcus sobrinus, Lactobacillus casei, Fusobacterium nucleatum, Atopobium rimae, and total bacteria was established by quantitative polymerase chain reaction. No signs of pain or restoration failure were observed. All therapies were effective in reducing the number of microorganisms, except for S. sobrinus. No statistical differences were observed among the protocols used. All therapies may be considered as effective modern approaches to minimal intervention for the management of deep primary caries treatment.

• Authors: Carolina Steiner-Oliveira, Priscila Larcher Longo, Ana Cecília Corrêa Aranha, Karen Müller Ramalho, Marcia Pinto Alves Mayer, Carlos de Paula Eduardo
• Journal: Journal of biomedical optics (J Biomed Opt) Vol. 20 Issue 10 Pg. 108003 (Oct 2015) ISSN: 1560-2281 [Electronic] United States
• PMID: 26502235 (Publication Type: Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Infective Agents
• Photosensitizing Agents

Topics

• Anti-Infective Agents (administration & dosage)
• Bacterial Infections (diagnosis, drug therapy, microbiology)
• Child
• Child, Preschool
• Combined Modality Therapy (methods)
• Dental Caries (diagnosis, drug therapy, microbiology)
• Female
• Humans
• Male
• Photochemotherapy (methods)
• Photosensitizing Agents (therapeutic use)
• Tooth, Deciduous (drug effects, microbiology, pathology)
• Treatment Outcome