The effects of RNA interference mediated VEGF gene silencing on biological behavior of renal cell carcinoma and transplanted renal tumor in nude mice.

Abstract

OBJECTIVE:

This study was to explore the effects of RNA interference mediated vascular endothelial growth factor (VEGF) gene silencing on biological behavior of renal cell carcinoma (RCC), transplanted renal tumor and angiogenesis in nude mice.

METHODS:

The specific siRNA sequence targeting VEGF were designed and synthesized to construct hVEGF-siRNA plasmid which was transfected into RCC 786-O cells. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used for the detection of VEGF gene expression and western blot was adopted for the examination of VEGF protein expression. The 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to detect cell growth as well as cell migration and invasion. The transplanted renal tumor models in nude mice were established, and the growth condition of nude mice, and VEGF protein expression in transplanted tumor slices and the microvessel density (MVD) were detected.

RESULTS:

The expression level of VEGF mRNA in VEGF-siRNA group was significant lower than that in the control group and negative group, suggesting that establishment of plasmid specifically inhibited the expression of VEGF gene The expression level of VEGF protein in VEGF-siRNA group was significant lower than that in the control group and negative group. VEGF gene silencing has the significant inhibition effects on proliferation, migration and invasion of RCC 786-O cells. The tumor weight, VEGF protein positive rate and MVD in VEGF-siRNA group were significant lower than those in negative group and blank group.

CONCLUSION:

The VEGF gene silencing could inhibit the cell proliferation, migration and invasion of RCC 786-O cells; inhibition of VEGF protein expression could prevent transplanted RCC growth and tumor angiogenesis.

• Authors: Qi Wang, Shuai Wang, Si-Qiao Sun, Zhi-Hua Cheng, Yang Zhang, Guang Chen, Meng Gu, Hai-Jun Yao, Zhong Wang, Juan Zhou, Yu-Bing Peng, Ming-Xi Xu, Ke Zhang, Xi-Wei Sun
• Journal: Cancer biomarkers : section A of Disease markers (Cancer Biomark) Vol. 16 Issue 1 Pg. 1-9 ( 2016) ISSN: 1875-8592 [Electronic] Netherlands
• PMID: 26484606 (Publication Type: Journal Article)

Chemical References

• RNA, Small Interfering
• Vascular Endothelial Growth Factor A

Topics

• Animals
• Carcinoma, Renal Cell (genetics, metabolism, pathology)
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation
• Disease Models, Animal
• Female
• Gene Expression
• Gene Silencing
• Humans
• Immunohistochemistry
• Kidney Neoplasms (genetics, metabolism, pathology)
• Mice
• Mice, Nude
• Neovascularization, Pathologic (genetics)
• RNA Interference
• RNA, Small Interfering (genetics)
• Vascular Endothelial Growth Factor A (genetics, metabolism)
• Xenograft Model Antitumor Assays