In the present study, we developed serological strategies using
immunoglobulin fractions obtained by
protein A chromatography to screen for
cervical cancer and
cervical intraepithelial neoplasia I (CIN I). The reactivities of the
immunoglobulins purified from sera of women with normal cytology, CIN I and
cervical cancer were compared in
enzyme-linked
immunosorbent assays (ELISA) and
enzyme-linked
lectin assays (ELLAs). To capture the
immunoglobulins, ELISAs and ELLAs were performed in
protein A immobilized microplates. The reactivity of
immunoglobulin in ELISA was in the increasing order normal cytology, CIN I and
cervical cancer, while that in ELLAs for detecting fucosylation was in the decreasing order normal cytology, CIN I and
cervical cancer. It was confirmed that women with CIN I were distinguishable from women with normal cytology or women with
cervical cancer in the ELISA or the ELLA for detecting fucosylation with considerable sensitivity and specificity. Women with
cervical cancer were also distinguishable from women with normal cytology with high sensitivity (ELISA: 97%, ELLA: 87%) and specificity (ELISA: 69%, ELLA: 72%). Moreover, the logistic regression model of the ELISA and the ELLA discriminated
cervical cancer from normal cytology with 93% sensitivity and 93% specificity. These results indicate that the ELISAs and the ELLAs have great potential as strategies for primary screening of
cervical cancer and CIN. It is expected that the ELISA and the ELLA can provide new insights to understand systemic changes of serum
immunoglobulins during
cervical cancer progression.