Antiseptic effect of vicenin-2 and scolymoside from Cyclopia subternata (honeybush) in response to HMGB1 as a late sepsis mediator in vitro and in vivo.

Abstract

Cyclopia subternata is a medicinal plant commonly used in traditional medicine to relieve pain. In this study, we investigated the antiseptic effects and underlying mechanisms of vicenin-2 and scolymoside, which are 2 active compounds from C. subternata that act against high mobility group box 1 (HMGB1)-mediated septic responses in human umbilical vein endothelial cells (HUVECs) and mice. The antiseptic activities of vicenin-2 and scolymoside were determined by measuring permeability, neutrophil adhesion and migration, and activation of proinflammatory proteins in HMGB1-activated HUVECs and mice. According to the results, vicenin-2 and scolymoside effectively inhibited lipopolysaccharide-induced release of HMGB1, and suppressed HMGB1-mediated septic responses such as hyperpermeability, the adhesion and migration of leukocytes, and the expression of cell adhesion molecules. In addition, vicenin-2 and scolymoside suppressed the production of tumor necrosis factor-α and interleukin 6, and activation of nuclear factor-κB and extracellular regulated kinases 1/2 by HMGB1. Collectively, these results indicate that vicenin-2 and scolymoside could be a potential therapeutic agents for the treatment of various severe vascular inflammatory diseases via inhibition of the HMGB1 signaling pathway.

• Authors: Wonhwa Lee, Eun-Kyung Yoon, Kyung-Min Kim, Dong Ho Park, Jong-Sup Bae
• Journal: Canadian journal of physiology and pharmacology (Can J Physiol Pharmacol) Vol. 93 Issue 8 Pg. 709-20 (Aug 2015) ISSN: 1205-7541 [Electronic] Canada
• PMID: 26243020 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Infective Agents, Local
• Anti-Inflammatory Agents
• Glucosides
• HMGB1 Protein
• HMGB1 protein, human
• Inflammation Mediators
• Lipopolysaccharides
• apigenin-6,8-di-C-glycopyranoside
• lipopolysaccharide, Escherichia coli O111 B4
• scolymoside
• Apigenin
• Luteolin

Topics

• Animals
• Anti-Infective Agents, Local (pharmacology)
• Anti-Inflammatory Agents (pharmacology)
• Apigenin (isolation & purification, pharmacology)
• Cell Adhesion (drug effects)
• Cell Membrane Permeability (drug effects)
• Cell Movement (drug effects)
• Cells, Cultured
• Coculture Techniques
• Cyclopia Plant (chemistry)
• Glucosides (isolation & purification, pharmacology)
• HMGB1 Protein (antagonists & inhibitors, pharmacology)
• Human Umbilical Vein Endothelial Cells (drug effects, metabolism)
• Humans
• Inflammation Mediators (metabolism)
• Lipopolysaccharides (pharmacology)
• Luteolin (isolation & purification, pharmacology)
• Male
• Mice, Inbred C57BL
• Neutrophils (drug effects, metabolism)
• Phytotherapy
• Plants, Medicinal
• Sepsis (drug therapy, metabolism, microbiology)
• Signal Transduction (drug effects)