MUC-1 expression in pleomorphic adenomas using two human milk fat globule protein membrane antibodies (HMFG-1 and HMFG-2).

Abstract

UNLABELLED:

Pleomorphic adenoma (PA) is the most common salivary gland tumor and its microscopic features and histogenesis are a matter of debate. Human milk fat globule protein membrane (HMFG) monoclonal antibodies (MoAbs) comprise a set of antibodies against the mucin 1 (MUC-1) protein detected in several salivary gland tumors.

OBJECTIVE:

The aim of this study was to assess the immunoexpression of the PA neoplastic cells to MUC-1 protein using HMFG-1 and HMFG-2 MoAbs, contrasting these results with those from normal salivary gland tissue.

MATERIAL AND METHODS:

Immunohistochemical detection of MUC-1 protein using HMFG-1 and HMFG-2 MoAbs was made in 5 mm thick, paraffin embedded slides, and the avidin-biotin method was used.

RESULTS:

Positivity to HMFG-1 and HMFG-2 MoAbs was found in ductal, squamous metaplastic and neoplastic myoepithelial cells, keratin pearls and intraductal mucous material. Two kinds of myoepithelial cells were identified: classic myoepithelial cells around ducts were negative to both MoAbs, and modified myoepithelial cells were positive to both MoAbs. This last cellular group of the analyzed tumors showed similar MUC-1 immunoexpression to ductal epithelial cells using both HMFG antibodies. Intraductal mucous secretion was also HMFG-1 and HMFG-2 positive.

CONCLUSIONS:

Our results showed there are two kinds of myoepithelial cells in PA. The first cellular group is represented by the different kinds of neoplastic myoepithelial cells and is HMFG-positive. The second one is HMFG-negative and represented by the neoplastic myoepithelial cells located around the ducts.

• Authors: Santa Ponce-Bravo, Constantino Ledesma-Montes, Maricela Garcés-Ortíz
• Journal: Journal of applied oral science : revista FOB (J Appl Oral Sci) 2015 May-Jun Vol. 23 Issue 3 Pg. 265-71 ISSN: 1678-7765 [Electronic] Brazil
• PMID: 26221920 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Biomarkers, Tumor
• Glycolipids
• Glycoproteins
• MUC1 protein, human
• Membrane Proteins
• Mucin-1
• milk fat globule

Topics

• Adenoma, Pleomorphic (chemistry, pathology)
• Antibodies, Monoclonal
• Biomarkers, Tumor (analysis)
• Case-Control Studies
• Glycolipids
• Glycoproteins
• Humans
• Immunohistochemistry
• Lipid Droplets
• Membrane Proteins
• Mucin-1 (analysis)
• Paraffin Embedding
• Reference Values
• Salivary Gland Neoplasms (chemistry, pathology)
• Salivary Glands (chemistry, metabolism)
• Staining and Labeling (methods)