Abstract |
The Shigella flexneri autotransporter protein IcsA is essential for intra- and intercellular spread, and icsA mutants are attenuated in several models. However, the pathogenic significance of the outer membrane protease IcsP, which orchestrates the polar distribution of IcsA on the bacterial surface, remains unclear. To further examine this point, we constructed icsP mutants in the two most commonly studied S. flexneri strains and evaluated their in vitro and in vivo performance relative to wild type. Both icsP mutants showed aberrant surface distribution of IcsA, but the in vitro consequences depended upon the cell line being used to assess bacterial motility and plaque formation. Evaluating the behaviour of the mutants in two mouse models suggested functional expression of icsP might limit bacterial persistence and the associated inflammation in host tissues, consistent with the findings in one of the three cell lines used.
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Authors | Elizabeth Ngoc Hoa Tran, Stephen R Attridge, Min Yan Teh, Renato Morona |
Journal | FEMS microbiology letters
(FEMS Microbiol Lett)
Vol. 362
Issue 12
Pg. fnv088
(Jun 2015)
ISSN: 1574-6968 [Electronic] England |
PMID | 26025071
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © FEMS 2015. All rights reserved. For permissions, please e-mail: [email protected]. |
Chemical References |
- Actins
- Aminoglycosides
- Bacterial Outer Membrane Proteins
- Bacterial Proteins
- DNA-Binding Proteins
- Transcription Factors
- virG protein, Shigella flexneri
- 1,2-diamino-1,2-N,N'-carbonyl-1,2-dideoxyglucose hydrate
- SopA protein, Bacteria
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Topics |
- Actins
(genetics)
- Aminoglycosides
- Animals
- Bacterial Outer Membrane Proteins
(genetics, metabolism)
- Bacterial Proteins
(genetics, metabolism)
- Caco-2 Cells
- Cell Line
- DNA-Binding Proteins
(metabolism)
- Dysentery, Bacillary
(microbiology)
- HeLa Cells
- Humans
- Male
- Mice
- Mice, Inbred BALB C
- Mutation
- Shigella flexneri
(genetics, pathogenicity)
- Transcription Factors
(metabolism)
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