The lectin from Musa paradisiaca binds with the capsid protein of tobacco mosaic virus and prevents viral infection.

Abstract

It has been demonstrated that the lectin from Musa paradisiaca (BanLec-1) could inhibit the cellular entry of human immunodeficiency virus (HIV). In order to evaluate its effects on tobacco mosaic virus (TMV), the banlec-1 gene was cloned and transformed into Escherichia coli and tobacco, respectively. Recombinant BanLec-1 showed metal ions dependence, and higher thermal and pH stability. Overexpression of banlec-1 in tobacco resulted in decreased leaf size, and higher resistance to TMV infection, which includes reduced TMV cellular entry, more stable chlorophyll contents, and enhanced antioxidant enzymes. BanLec-1 was found to bind directly to the TMV capsid protein in vitro, and to inhibit TMV infection in a dose-dependent manner. In contrast to limited prevention in vivo, purified rBanLec-1 exhibited more significant effects on TMV infection in vitro. Taken together, our study indicated that BanLec-1 could prevent TMV infection in tobacco, probably through the interaction between BanLec-1 and TMV capsid protein.

• Authors: Xiao-Yu Liu, Huan Li, Wei Zhang
• Journal: Biotechnology, biotechnological equipment (Biotechnol Biotechnol Equip) Vol. 28 Issue 3 Pg. 408-416 (May 04 2014) ISSN: 1310-2818 [Print] England
• PMID: 26019527 (Publication Type: Journal Article)