MiRNA profile of osteosarcoma with CD117 and stro-1 expression: miR-1247 functions as an onco-miRNA by targeting MAP3K9.

Abstract

microRNAs (miRNA) are regulators of gene expression, but little is known about miRNA expression profiles in stem cells of osteosarcoma (OS). C117 and Stro-1 are known stem cell markers of OS. In the study, CD117 and stro-1 positive (CD117(+)stro-1(+)) and CD117 and stro-1 negative (CD117(-)stro-1(-)) cells were isolated from MG63 cells CD117(+)stro-1(+) cells showed more metastatic ability and stem cell formation rate than CD117(-)stro-1(-) ones. To find the difference between CD117(+)stro-1(+) and CD117(-)stro-1(-) cells, the miRNA expression profile was examined using DNA microarray. MicroRNAs were differentially expressed in osteosarcoma cells with CD117(+)stro-1(+) and CD117(-)stro-1(-). The significant miRNAs included miR-15a, miR-302a, miR-423-5p, miR-1247, miR-1243 and others, which were confirmed by real time RT-PCR. The significant down-regulated miR-1247 was confirmed that was a potential tumor suppressor by targeting MAP3K9. Our results indicated that dysregulation of miRNAs is involved in osteosarcoma and miR-1247 plays an important role in progression of osteosarcoma.

• Authors: Fuyou Zhao, Jie Lv, Huaiyong Gan, Yumei Li, Ri Wang, Haoran Zhang, Qiong Wu, Yuqing Chen
• Journal: International journal of clinical and experimental pathology (Int J Clin Exp Pathol) Vol. 8 Issue 2 Pg. 1451-8 ( 2015) ISSN: 1936-2625 [Electronic] United States
• PMID: 25973030 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Surface
• MIRN1247 microRNA, human
• MicroRNAs
• STRO-1 antigen, human
• Proto-Oncogene Proteins c-kit
• MAP Kinase Kinase Kinases
• MAP3K9 protein, human

Topics

• Antigens, Surface (biosynthesis)
• Blotting, Western
• Bone Neoplasms (genetics, pathology)
• Cell Line, Tumor
• Cell Separation
• Flow Cytometry
• Gene Expression Regulation, Neoplastic (genetics)
• Humans
• MAP Kinase Kinase Kinases (metabolism)
• MicroRNAs (genetics)
• Neoplastic Stem Cells (pathology)
• Oligonucleotide Array Sequence Analysis
• Osteosarcoma (genetics, pathology)
• Proto-Oncogene Proteins c-kit (biosynthesis)
• Real-Time Polymerase Chain Reaction
• Transcriptome