HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Surface-enhanced Raman scattering (SERS)-active gold nanochains for multiplex detection and photodynamic therapy of cancer.

Abstract
Multifunctional nanomedicine holds considerable promise as the next generation of medicine that will enable early detection of diseases, as well as simultaneous monitoring and therapy with minimal toxicity. In particular, surface-enhanced Raman scattering (SERS) technology with high sensitivity and multiplexing capabilities is emerging as a powerful alternative for identifying specific biological targets in live cells. In this paper, we present the synthesis of SERS-active gold nanochains (AuNCs) as a potential theranostic system for multiplex detection and photodynamic therapy (PDT) of cancer. AuNCs were prepared by a simple physical mixing method to assemble citrate-stabilized gold nanoparticles into nanochains using hyaluronic acid and hydrocaffeic acid (HA-HCA) conjugates as templates. In addition, Raman reporters and photosensitizers (PSs) were conjugated onto the surface of the AuNCs for multiplex detection and PDT action. After mixing with HA-HCA conjugates, citrate-stabilized gold nanoparticles formed the AuNC structure, and AuNC length was controlled by the HCA conjugation ratio in the HA-HCA conjugates. AuNCs exhibited maximal absorption in the near-infrared (NIR) spectral region and effective SERS property. Confocal microscopy, flow cytometry, Raman spectroscopy and Bio-TEM measurements were used to determine cellular uptake of the Raman reporter, PS and AuNCs in HeLa cells. AuNCs conjugated with Raman reporter and PS (HA-HCAn-Au-Pheo-NPT) showed more than 99% cellular uptake and exhibited excellent phototoxicity even at low PS concentrations compared with free PS after laser irradiation. This SERS-active AuNC (HA-HCAn-Au-Pheo-NPT) shows promise for applications in theranostics, integrating SERS imaging and PDT.
AuthorsLinlin Zhao, Tae-Hyun Kim, Hae-Won Kim, Jin-Chul Ahn, So Yeon Kim
JournalActa biomaterialia (Acta Biomater) Vol. 20 Pg. 155-164 (Jul 2015) ISSN: 1878-7568 [Electronic] England
PMID25848726 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Chemical References
  • Caffeic Acids
  • Solutions
  • Gold
  • Hyaluronic Acid
  • caffeic acid
Topics
  • Caffeic Acids (chemistry)
  • Dynamic Light Scattering
  • Flow Cytometry
  • Gold (chemistry)
  • HeLa Cells
  • Humans
  • Hyaluronic Acid (chemistry)
  • Metal Nanoparticles (chemistry, ultrastructure)
  • Microscopy, Confocal
  • Neoplasms (diagnosis, drug therapy)
  • Photochemotherapy
  • Solutions
  • Spectroscopy, Fourier Transform Infrared
  • Spectrum Analysis, Raman (methods)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: