Antihyperlipidemic effect of Cyclocarya paliurus (Batal.) Iljinskaja extract and inhibition of apolipoprotein B48 overproduction in hyperlipidemic mice.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Cyclocarya paliurus (CP) Batal., the sole species in its genus, is a native plant to China. As a traditional Chinese folk medicine, the tree leaves have been widely used for the treatment of metabolic disorders, including hyperlipidemia, obesity, diabetes and hypertension.

AIM OF THE STUDY:

The study aimed to evaluate the antihyperlipidemic effect of CP ethanol extract, as well as its inhibitory activity on apolipoproteinB48 (apoB48), in normal and hyperlipidemic mice.

MATERIALS AND METHODS:

The antihyperlipidemic effect of CP was evaluated in hyperlipidemic mice induced by high-fat diet for 4 weeks. CP ethanol extract (0.37, 0.75 and 1.5g/kg/day) was orally administrated once daily. Lipids and antioxidant profiles, including total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), together with the indices of hepatic and renal functions were examined. RT-qPCR and western blotting were used to analysis the expression levels of tumor necrosis factor (TNF-α), total- and triglyceride-rich apoB48 (TRL-apoB48), as well as the phosphorylation of the mitogen-activatein kinase (MAPK).

RESULTS:

CP as well as simvastatin remarkably lowered the levels of TC, TG, LDL-C and MDA, and at the same time, elevated the HDL-C, SOD and GSH-Px in high-fat diet mice. It also decreased the serum concentration of total- and TRL-apoB48 in the fasting state. CP inhibited TNF-α expression and phosphorylation level of MAPK. Furthermore, the HE staining of liver and kidney, together with hepatic and renal function analysis showed hepato- and renoprotective activities of CP.

CONCLUSIONS:

These results suggested that CP possesses beneficial potentials for use in treating hyperlipidemia and the underlying lipid-lowering mechanism might associate with a down-regulation of the intestinal-associated lipoprotein apoB48, which may provide evidence about its practical application for treating hyperlipidemia and its complications.

• Authors: Yonglan Ma, Cuihua Jiang, Nan Yao, Yue Li, Qingqing Wang, Shengzuo Fang, Xulan Shang, Ming Zhao, Chuntao Che, Yicheng Ni, Jian Zhang, Zhiqi Yin
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 166 Pg. 286-96 (May 26 2015) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 25794806 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Antioxidants
• Apolipoprotein B-48
• Cholesterol, HDL
• Cholesterol, LDL
• Drugs, Chinese Herbal
• Hypolipidemic Agents
• Lipids
• Plant Extracts
• Triglycerides
• Tumor Necrosis Factor-alpha
• Malondialdehyde
• Glutathione Peroxidase
• Superoxide Dismutase
• Mitogen-Activated Protein Kinase Kinases

Topics

• Animals
• Antioxidants (metabolism)
• Apolipoprotein B-48 (antagonists & inhibitors)
• Cholesterol, HDL (blood)
• Cholesterol, LDL (blood)
• Diet, High-Fat (adverse effects)
• Drugs, Chinese Herbal (chemistry, pharmacology)
• Ethnopharmacology (methods)
• Glutathione Peroxidase (blood)
• Hyperlipidemias (blood, drug therapy, metabolism)
• Hypolipidemic Agents (chemistry, pharmacology)
• Juglandaceae (chemistry)
• Kidney (drug effects, metabolism)
• Lipids (blood)
• Liver (drug effects, metabolism)
• Male
• Malondialdehyde (blood)
• Mice
• Mitogen-Activated Protein Kinase Kinases (metabolism)
• Plant Extracts (chemistry, pharmacology)
• Plant Leaves (chemistry)
• Superoxide Dismutase (blood)
• Triglycerides (blood)
• Tumor Necrosis Factor-alpha (metabolism)