High-
carbohydrate diets have been associated with β-cell strain,
dyslipidemia, and endothelial dysfunction. We examined how β-cell and endothelial function adapt to
carbohydrate overloading and the influence of
insulin resistance. On sequential days in randomized order, nondiabetic subjects (classified as
insulin-sensitive [IS] [n = 64] or
insulin-resistant [IR] [n = 79] by euglycemic clamp) received four mixed meals over 14 h with either standard (300 kcal) or double
carbohydrate content. β-Cell function was reconstructed by mathematical modeling; brachial artery flow-mediated dilation (FMD) was measured before and after each meal. Compared with IS, IR subjects showed higher glycemia and
insulin hypersecretion due to greater β-cell
glucose and rate sensitivity; potentiation of insulin secretion, however, was impaired. Circulating
free fatty acids (FFAs) were less suppressed in IR than IS subjects. Baseline FMD was reduced in IR, and postprandial FMD attenuation occurred after each meal, particularly with high
carbohydrate, similarly in IR and IS. Throughout the two study days, higher FFA levels were significantly associated with lower (
incretin-induced) potentiation and impaired FMD. In nondiabetic individuals, enhanced
glucose sensitivity and potentiation upregulate the
insulin secretory response to
carbohydrate overloading. With
insulin resistance, this adaptation is impaired. Defective suppression of endogenous FFA is one common link between impaired potentiation and vascular endothelial dysfunction.