Oral squamous cancer cell exploits hnRNP A1 to regulate cell cycle and proliferation.

Abstract

Oral squamous cell carcinoma (OSCC) is a common human malignant tumor with high mortality. So far, the molecular pathogenesis of OSCC remains largely unclear. Heterogeneous nuclear ribonucleoprotein (hnRNP) A1 is an important multi-function splicing factor and closely related to tumorigenesis. hnRNP A1 is overexpressed in various tumors, and promotes aerobic glycolysis and elongation of telomere, but the function of hnRNP A1 in cell cycle and proliferation remains unclear. We found that hnRNP A1 was overexpressed in OSCC tissues, and was required for the growth of OSCC cells. Moreover, hnRNP A1 was highly expressed in the G2/M cell cycle phase. Knockdown of hnRNP A1 induced G2/M arrest. DNA microarray assay result showed that hnRNP A1 regulated the expression of a number of target genes associated with G2/M phase. Moreover, hnRNP A1 controlled the alternative splicing of CDK2 exon 5. These findings suggested that hnRNP A1 plays key roles in the regulation of cell cycle progression and pathogenesis of OSCC.

• Authors: Cheng Yu, Jihua Guo, Yu Liu, Jun Jia, Rong Jia, Mingwen Fan
• Journal: Journal of cellular physiology (J Cell Physiol) Vol. 230 Issue 9 Pg. 2252-61 (Sep 2015) ISSN: 1097-4652 [Electronic] United States
• PMID: 25752295 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2015 Wiley Periodicals, Inc.

Chemical References

• Heterogeneous Nuclear Ribonucleoprotein A1
• Heterogeneous-Nuclear Ribonucleoprotein Group A-B
• Neoplasm Proteins
• CDK2 protein, human
• Cyclin-Dependent Kinase 2

Topics

• Alternative Splicing (genetics)
• Carcinoma, Squamous Cell (genetics, pathology)
• Cell Cycle (genetics)
• Cell Proliferation (genetics)
• Cell Transformation, Neoplastic (genetics)
• Cyclin-Dependent Kinase 2 (genetics)
• Gene Expression Regulation, Neoplastic
• Heterogeneous Nuclear Ribonucleoprotein A1
• Heterogeneous-Nuclear Ribonucleoprotein Group A-B (genetics)
• Humans
• Mouth Neoplasms (genetics, pathology)
• Neoplasm Proteins (biosynthesis)
• Primary Cell Culture
• RNA Splicing (genetics)