Abstract |
Contagious ovine digital dermatitis (CODD) is an important foot disease in sheep, with significant animal welfare and economic implications. It is thought that CODD emerged from bovine digital dermatitis ( BDD) via treponemal bacteria. With wildlife species such as elk now suffering a CODD-like disease, it is imperative to clarify these disease etiologies. A large investigation into treponemal association with CODD is warranted. CODD lesions (n = 58) and healthy sheep foot tissues (n = 56) were analyzed by PCR for the three BDD-associated Treponema phylogroups and two other lameness-associated bacteria, Dichelobacter nodosus and Fusobacterium necrophorum. Spirochete culture was also attempted on CODD lesions. "Treponema medium/Treponema vincentii-like," "Treponema phagedenis-like," and Treponema pedis spirochetes were identified in 39/58 (67%), 49/58 (85%), and 41/58 (71%) of CODD lesions, respectively. One or more BDD-associated Treponema phylogroups were detected in 100% of CODD lesions. Healthy foot tissues did not amplify BDD-associated Treponema phylogroup DNA. D. nodosus and F. necrophorum were present in 34/58 (59%) and 41/58 (71%) of CODD lesions and 22/56 (39%) and 5/56 (9%) of healthy foot tissues, respectively. Thirty-two spirochetes were isolated from CODD lesions, with representatives clustering with, and indistinguishable from, each of the three BDD-associated Treponema phylogroups based on 16S rRNA gene comparisons. This study for the first time demonstrates a high-level association for BDD treponeme phylogroups in CODD and their absence from healthy tissues, supporting the hypothesis that BDD treponemes play a primary causative role in CODD and confirming that the specific PCR assays are an effective differential diagnostic tool for CODD.
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Authors | L E Sullivan, S R Clegg, J W Angell, K Newbrook, R W Blowey, S D Carter, J Bell, J S Duncan, D H Grove-White, R D Murray, N J Evans |
Journal | Journal of clinical microbiology
(J Clin Microbiol)
Vol. 53
Issue 5
Pg. 1628-38
(May 2015)
ISSN: 1098-660X [Electronic] United States |
PMID | 25740778
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015, American Society for Microbiology. All Rights Reserved. |
Chemical References |
- DNA, Bacterial
- DNA, Ribosomal
- RNA, Ribosomal, 16S
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Topics |
- Animals
- Cattle
- Cluster Analysis
- DNA, Bacterial
(chemistry, genetics)
- DNA, Ribosomal
(chemistry, genetics)
- Dichelobacter nodosus
(isolation & purification)
- Digital Dermatitis
(microbiology)
- Fusobacterium necrophorum
(isolation & purification)
- Molecular Sequence Data
- Phylogeny
- Polymerase Chain Reaction
- RNA, Ribosomal, 16S
(genetics)
- Sequence Analysis, DNA
- Sheep, Domestic
- Treponema
(isolation & purification)
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