9-Oxo-(10E,12E)-octadecadienoic acid, a cytotoxic fatty acid ketodiene isolated from eggplant calyx, induces apoptosis in human ovarian cancer (HRA) cells.

Abstract

9-Oxo-(10E,12E)-octadecadienoic acid (9-EE-KODE), which is isolated from the calyx of eggplants, exhibits cytotoxic activity against human ovarian cancer (HRA) cells. The aim of the present study is to clarify the action mechanism of 9-EE-KODE leading to cell death. After the treatment of 9-EE-KODE in HRA cells, we found intracellular DNA fragmentation, surface-exposure of phosphatidylserine in the outer cell membrane, and increased caspase-3/7 activities in the HRA cells. The dissipation of mitochondrial membrane potential, release of cytochrome c from mitochondria to cytosol, down-regulation of Bcl-2, and up-regulation of Bax levels were also found in 9-EE-KODE-treated cells in a dose-dependent manner. These results demonstrated that 9-EE-KODE induced apoptosis in HRA cells via the mitochondrial regulation pathway.

• Authors: Baiyang Zhao, Yutaka Tomoda, Hajime Mizukami, Toshiaki Makino
• Journal: Journal of natural medicines (J Nat Med) Vol. 69 Issue 3 Pg. 296-302 (Jul 2015) ISSN: 1861-0293 [Electronic] Japan
• PMID: 25724148 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents, Phytogenic
• Linolenic Acids
• 9-oxo-10,12-octadecadienoic acid
• Cytochromes c
• CASP3 protein, human
• Caspase 3

Topics

• Antineoplastic Agents, Phytogenic (isolation & purification, pharmacology)
• Apoptosis (drug effects)
• Caspase 3 (metabolism)
• Cell Line, Tumor
• Cytochromes c (metabolism)
• DNA Fragmentation
• Drug Screening Assays, Antitumor
• Female
• Flowers (chemistry)
• Humans
• Linolenic Acids (isolation & purification, pharmacology)
• Membrane Potential, Mitochondrial (drug effects)
• Mitochondria (metabolism)
• Ovarian Neoplasms (drug therapy)
• Solanum melongena (chemistry)