Recognition of multiepitope dendrimeric antigens by human immunoglobulin E.

Abstract

In vitro drug allergy tests have limited sensitivity, partly due to a poor understanding of the immunological recognition of in vitro drug-protein conjugates. We have designed and synthesized multivalent mono- and bi-epitope dendrimeric antigen (DeAn) conjugates and studied their chemical and tridimensional structures. We describe differences in the spatial distribution and conformation of these conjugated epitopes for the first time: a partially hidden benzylpenicilloyl and a more exposed amoxicilloyl. Our data suggest that DeAn conjugates provide a useful model for studying IgE recognition in patients who suffer from an allergic reaction to benzylpenicillin and/or amoxicillin. 1D and 2D NMR, MDS and immunochemical studies provide evidence that both antigen composition and tridimensional distribution play key roles in IgE-antigen recognition. Bi-epitope DeAn conjugates could potentially allow the diagnosis of patients allergic to any of these two drugs with a single test and represent the basis for a broadly-applicable in vitro assay. From the clinical editor: The prevalence of allergic drug reactions is rising and there is an imperative need to identify patients at risk. In this interesting and important article, the authors developed a novel method for detecting drug specific IgE antibodies, responsible for allergic reactions, by using multivalent mono- and bi-epitope Dendrimeric Antigen (DeAn) conjugates. The continued success of this research may pave way of eventual development of a simple diagnostic test.

• Authors: María I Montañez, Francisco Najera, Cristobalina Mayorga, Antonio J Ruiz-Sanchez, Yolanda Vida, Daniel Collado, Miguel Blanca, María J Torres, Ezequiel Perez-Inestrosa
• Journal: Nanomedicine : nanotechnology, biology, and medicine (Nanomedicine) Vol. 11 Issue 3 Pg. 579-88 (Apr 2015) ISSN: 1549-9642 [Electronic] United States
• PMID: 25661921 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Inc. All rights reserved.

Chemical References

• Dendrimers
• Epitopes
• PAMAM Starburst
• Immunoglobulin E
• Amoxicillin
• Penicillin G

Topics

• Amoxicillin (chemistry, immunology)
• Dendrimers (chemistry)
• Drug Hypersensitivity (immunology)
• Epitopes (chemistry, immunology)
• Female
• Humans
• Immunoglobulin E (chemistry, immunology)
• Male
• Penicillin G (chemistry, immunology)