Pea (Pisum sativum L.) seed albumin extracts show anti-inflammatory effect in the DSS model of mouse colitis.

Abstract

SCOPE:

This study investigates the preventive effects of two pea (Pisum sativum) seed albumin extracts, either in the presence (pea seed extract [PSE]) or absence (albumin fraction from PSE [AF-PSE]) of soluble polysaccharides, in the dextran sodium sulfate (DSS) induced colitis in mice.

METHODS AND RESULTS:

Male C57BL/6J mice were assigned to five groups: one noncolitic and four colitic. Colitis was induced by incorporating DSS (3.5%) in the drinking water for 4 days, after which DSS was removed. Treated groups received orally PSE (15 g/kg⋅day), or AF-PSE (1.5 g/kg⋅day), or pure soy Bowman-Birk inhibitor (BBI; 50 mg/kg⋅day), starting 2 wk before colitis induction, and maintained for 9 days after. All treated groups showed intestinal anti-inflammatory effect, evidenced by reduced microscopic histological damage in comparison with untreated colitic mice. The treatments ameliorated the colonic mRNA expression of different proinflammatory markers: cytokines, inducible enzymes, metalloproteinases, adhesion molecules, and toll-like receptors, as well as proteins involved in maintaining the epithelial barrier function. Furthermore, the administration of PSE, AF-PSE, or soy BBI restored bacterial counts, partially or totally, to values in healthy mice.

CONCLUSION:

PSE and AF-PSE ameliorated DSS-induced damage to mice, their effects being due, at least partially, to the presence of active BBI.

• Authors: Ma Pilar Utrilla, Ma Jesus Peinado, Raquel Ruiz, Alba Rodriguez-Nogales, Francesca Algieri, Ma Elena Rodriguez-Cabezas, Alfonso Clemente, Julio Galvez, Luis A Rubio
• Journal: Molecular nutrition & food research (Mol Nutr Food Res) Vol. 59 Issue 4 Pg. 807-19 (Apr 2015) ISSN: 1613-4133 [Electronic] Germany
• PMID: 25626675 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Albumins
• Anti-Inflammatory Agents
• Interleukin-1beta
• Interleukin-6
• RNA, Messenger
• Tumor Necrosis Factor-alpha
• Interleukin-12
• Interferon-gamma
• Dextran Sulfate
• Ptgs2 protein, mouse
• Cyclooxygenase 2

Topics

• Albumins (pharmacology)
• Animals
• Anti-Inflammatory Agents (pharmacology)
• Cecum (drug effects, microbiology)
• Colitis (chemically induced, drug therapy)
• Colon (metabolism, microbiology)
• Cyclooxygenase 2 (genetics, metabolism)
• Dextran Sulfate (adverse effects)
• Disease Models, Animal
• Gastrointestinal Microbiome
• Interferon-gamma (genetics, metabolism)
• Interleukin-12 (genetics, metabolism)
• Interleukin-1beta (genetics, metabolism)
• Interleukin-6 (genetics, metabolism)
• Intestinal Mucosa (metabolism)
• Intestines (drug effects)
• Male
• Mice
• Mice, Inbred C57BL
• Pisum sativum (chemistry)
• RNA, Messenger (genetics, metabolism)
• Seeds (chemistry)
• Tumor Necrosis Factor-alpha (genetics, metabolism)