Differential reliance on autophagy for protection from HSV encephalitis between newborns and adults.

Abstract

Newborns are more susceptible to severe disease from infection than adults, with maturation of immune responses implicated as a major factor. The type I interferon response delays mortality and limits viral replication in adult mice in a model of herpes simplex virus (HSV) encephalitis. We found that intact type I interferon signaling did not control HSV disease in the neonatal brain. However, the multifunctional HSV protein γ34.5 involved in countering type I interferon responses was important for virulence in the brain in both age groups. To investigate this observation further, we studied a specific function of γ34.5 which contributes to HSV pathogenesis in the adult brain, inhibition of the cellular process of autophagy. Surprisingly, we found that the beclin binding domain of γ34.5 responsible for inhibiting autophagy was dispensable for HSV disease in the neonatal brain, as infection of newborns with the deletion mutant decreased time to mortality compared to the rescue virus. Additionally, a functional beclin binding domain in HSV γ34.5 did not effectively inhibit autophagy in the neonate, unlike in the adult. Type I IFN responses promote autophagy in adult, a finding we confirmed in the adult brain after HSV infection; however, in the newborn brain we observed that autophagy was activated through a type I IFN-independent mechanism. Furthermore, autophagy in the wild-type neonatal mouse was associated with increased apoptosis in infected regions of the brain. Observations in the mouse model were consistent with those in a human case of neonatal HSV encephalitis. Our findings reveal age-dependent differences in autophagy for protection from HSV encephalitis, indicating developmental differences in induction and regulation of this innate defense mechanism after HSV infection in the neonatal brain.

• Authors: Douglas R Wilcox, Nitin R Wadhwani, Richard Longnecker, William J Muller
• Journal: PLoS pathogens (PLoS Pathog) Vol. 11 Issue 1 Pg. e1004580 (Jan 2015) ISSN: 1553-7374 [Electronic] United States
• PMID: 25569138 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Topics

• Animals
• Animals, Newborn
• Apoptosis (physiology)
• Autophagy (physiology)
• Autopsy
• Encephalitis, Herpes Simplex (congenital, pathology, physiopathology)
• Female
• Herpes Simplex (pathology, physiopathology)
• Humans
• Infant
• Infant, Newborn
• Male
• Mice
• Mice, Knockout
• Pregnancy Complications, Infectious (pathology, physiopathology)