Human adipose tissue-derived mesenchymal stem cells inhibit melanoma growth in vitro and in vivo.

Abstract

BACKGROUND/AIM:

The effects of adipose tissue-derived mesenchymal stem cells (AT-MSCs) on the growth of human malignancies, including melanoma, are controversial and the underlying mechanisms are not yet-well understood. The aim of the present study was to investigate the in vitro and in vivo anti-tumor effects of human AT-MSCs on human melanoma.

MATERIALS AND METHODS:

The inhibitory effect of AT-MSC-conditioned medium (AT-MSC-CM) on the growth of A375SM and A375P (human melanoma) cells was evaluated using a cell viability assay. Cell-cycle arrest and apoptosis in melanoma cells were investigated by flow cytometry and western blot analysis. To evaluate the in vivo anti-tumor effect of AT-MSCs, CM-DiI-labeled AT-MSCs were circumtumorally injected in tumor-bearing athymic mice and tumor size was measured.

RESULTS:

AT-MSC-CM inhibited melanoma growth by altering cell-cycle distribution and inducing apoptosis in vitro. AT-MSCs suppressed tumor growth in tumor-bearing athymic mice and fluorescence analysis showed that AT-MSCs migrated efficiently to tumor tissues.

CONCLUSION:

AT-MSCs inhibit the growth of melanoma suggesting promise as a novel therapeutic agent for melanoma.

• Authors: Jin-Ok Ahn, Ye-Rin Coh, Hee-Woo Lee, Il-Seob Shin, Sung-Keun Kang, Hwa-Young Youn
• Journal: Anticancer research (Anticancer Res) Vol. 35 Issue 1 Pg. 159-68 (Jan 2015) ISSN: 1791-7530 [Electronic] Greece
• PMID: 25550547 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

Topics

• Animals
• Apoptosis
• Cell Line, Tumor
• Cell Proliferation
• Cell Survival
• Coculture Techniques
• Female
• Humans
• Melanoma (pathology, therapy)
• Mesenchymal Stem Cell Transplantation
• Mesenchymal Stem Cells (physiology)
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Neoplasm Transplantation
• Subcutaneous Fat, Abdominal (cytology)
• Tumor Burden