Abstract | BACKGROUND: METHODS AND RESULTS: Fifteen-week-old mice (n=66) were infused with angiotensin II for 4 weeks to induce AAA. Saline-infused mice (n=16) served as control. The surviving animals underwent in vivo MMP-targeted micro-single photon emission computed tomographic/computed tomographic imaging, using RP805, a technetium-99m-labeled MMP-specific tracer, followed by ex vivo planar imaging, morphometry, and gene expression analysis. RP805 uptake in suprarenal aorta on micro-single photon emission computed tomographic images was significantly higher in animals with AAA when compared with angiotensin II-infused animals without AAA or control animals. CD68 expression and MMP activity were increased in AAA, and significant correlations were noted between RP805 uptake and CD68 expression or MMP activity but not aortic diameter. A group of angiotensin II-infused animals (n=24) were imaged at 1 week and were followed up for additional 3 weeks. RP805 uptake in suprarenal aorta at 1 week was significantly higher in mice that later developed rupture or AAA. Furthermore, tracer uptake at 1 week correlated with aortic diameter at 4 weeks. CONCLUSIONS:
MMP-targeted imaging reflects vessel wall inflammation and can predict future aortic expansion or rupture in murine AAA. If confirmed in humans, this may provide a new paradigm for AAA risk stratification.
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Authors | Reza Golestani, Mahmoud Razavian, Lei Nie, Jiasheng Zhang, Jae-Joon Jung, Yunpeng Ye, Michelle de Roo, Koen Hilgerink, Chi Liu, Simon P Robinson, Mehran M Sadeghi |
Journal | Circulation. Cardiovascular imaging
(Circ Cardiovasc Imaging)
Vol. 8
Issue 1
(Jan 2015)
ISSN: 1942-0080 [Electronic] United States |
PMID | 25550400
(Publication Type: Evaluation Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)
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Copyright | © 2014 American Heart Association, Inc. |
Chemical References |
- Antigens, CD
- Antigens, Differentiation, Myelomonocytic
- Biomarkers
- CD68 protein, mouse
- Radiopharmaceuticals
- Angiotensin II
- Matrix Metalloproteinases
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Topics |
- Angiotensin II
- Animals
- Antigens, CD
(metabolism)
- Antigens, Differentiation, Myelomonocytic
(metabolism)
- Aorta, Abdominal
(diagnostic imaging, metabolism, pathology)
- Aortic Aneurysm, Abdominal
(chemically induced, diagnosis, metabolism)
- Aortic Rupture
(diagnosis, etiology, metabolism)
- Aortography
- Biomarkers
(metabolism)
- Disease Models, Animal
- Disease Progression
- Enzyme Activation
- Feasibility Studies
- Male
- Matrix Metalloproteinases
(metabolism)
- Mice, Inbred C57BL
- Mice, Transgenic
- Molecular Imaging
(methods)
- Multimodal Imaging
- Predictive Value of Tests
- Radiopharmaceuticals
- Risk Assessment
- Risk Factors
- Time Factors
- Tomography, Emission-Computed, Single-Photon
- Tomography, X-Ray Computed
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