Levels of blood CD1c+ mDC1 and CD1chi mDC1 subpopulation reflect disease activity in noninfectious uveitis.

Abstract

PURPOSE:

Myeloid dendritic cells (mDCs) play an important role in autoimmune diseases. However, the role of blood CD1c(+) myeloid dendritic cells 1 (mDC1s), the subset of human blood mDCs, is not well understood in noninfectious uveitis.

METHODS:

Fresh peripheral blood samples from human noninfectious uveitis patients (n = 32) and healthy controls (HCs) (n = 64) were stained with FITC-Lineage 1 (Lin1), PERCP-HLADR, and PE-CD1c antibodies. The levels of mDC1 were quantified by using flow cytometric analysis. Longitudinal data from patients (n = 16) were analyzed to correlate the levels of mDC1 with disease activity.

RESULTS:

Blood CD1c(+) mDC1 and its subpopulation, CD1c(hi) mDC1, were increased in uveitis patients compared with HCs. Longitudinal data demonstrated that both the CD1c(+) mDC1 and CD1c(hi) mDC1 subpopulation reflected a dynamic change in clinical uveitis activity: CD1c expression was increased in active uveitis but decreased when uveitis became inactive.

CONCLUSIONS:

Given these observations, an alteration in blood CD1c(+) mDC1 and the CD1c(hi) mDC1 subpopulation could be a potential biomarker to monitor clinical uveitis activity within patients.

• Authors: Ping Chen, William Tucker, Susan Hannes, Baoying Liu, Han Si, Ankur Gupta, Richard W J Lee, H Nida Sen, Robert B Nussenblatt
• Journal: Investigative ophthalmology & visual science (Invest Ophthalmol Vis Sci) Vol. 56 Issue 1 Pg. 346-52 (Dec 16 2014) ISSN: 1552-5783 [Electronic] United States
• PMID: 25515573 (Publication Type: Journal Article, Research Support, N.I.H., Intramural)
• Copyright: Copyright 2015 The Association for Research in Vision and Ophthalmology, Inc.

Chemical References

• Adaptor Proteins, Signal Transducing
• Antigens, CD1
• Biomarkers
• CD1C protein, human
• Cell Cycle Proteins
• Glycoproteins
• MDC1 protein, human
• Nuclear Proteins
• Trans-Activators

Topics

• Adaptor Proteins, Signal Transducing
• Adolescent
• Adult
• Aged
• Antigens, CD1 (blood, immunology)
• Autoimmune Diseases (blood, immunology)
• Biomarkers (blood)
• Cell Cycle Proteins
• Child
• Dendritic Cells (immunology)
• Female
• Flow Cytometry
• Glycoproteins (blood, immunology)
• Humans
• Immunity, Cellular
• Male
• Middle Aged
• Myeloid Cells (immunology)
• Nuclear Proteins (blood, immunology)
• Trans-Activators (blood, immunology)
• Uveitis (blood, immunology)
• Young Adult