ATOH8: a novel marker in human muscle fiber regeneration.

Abstract

Regenerating muscle fibers emerge from quiescent satellite cells, which differentiate into mature multinuclear myofibers upon activation. It has recently been found that ATOH8, a bHLH transcription factor, is regulated during myogenic differentiation. In this study, expression and localization of ATOH8, the other well-described regeneration markers, vimentin, nestin and neonatal myosin, and the satellite cell marker Pax7 were analyzed on protein level in human myopathy samples by immunofluorescence studies. On mRNA level, expression levels of ATOH8 and vimentin were studied by quantitative real-time PCR. ATOH8 is expressed in activated satellite cells and proliferating myoblasts of human skeletal muscle tissue. Quantitative analyses of ATOH8+, Pax7+, vimentin+, nestin+ and neonatal myosin+ muscle fibers showed the highest amount of regenerating muscle fibers in inflammatory myopathies, followed by muscular dystrophy. The relative co-expression of ATOH8 with the above-mentioned markers did not vary among the disorders. These results show that the novel regeneration marker ATOH8 contributes to muscle cell differentiation in healthy and diseased human muscle tissue.

• Authors: Anne-K Güttsches, Ajeesh Balakrishnan-Renuka, Rudolf André Kley, Martin Tegenthoff, Beate Brand-Saberi, Matthias Vorgerd
• Journal: Histochemistry and cell biology (Histochem Cell Biol) Vol. 143 Issue 5 Pg. 443-52 (May 2015) ISSN: 1432-119X [Electronic] Germany
• PMID: 25514850 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ATOH8 protein, human
• Basic Helix-Loop-Helix Transcription Factors
• NES protein, human
• Nestin
• PAX7 Transcription Factor
• PAX7 protein, human
• RNA, Messenger
• Vimentin
• Myosins

Topics

• Adult
• Aged
• Aged, 80 and over
• Basic Helix-Loop-Helix Transcription Factors (genetics, metabolism)
• Case-Control Studies
• Cell Differentiation
• Cell Proliferation
• Female
• Fluorescent Antibody Technique
• Humans
• Male
• Middle Aged
• Muscle Fibers, Skeletal (metabolism, pathology)
• Muscular Diseases (genetics, metabolism, pathology, physiopathology)
• Muscular Dystrophies (metabolism, pathology, physiopathology)
• Myoblasts, Skeletal (metabolism, pathology)
• Myosins (metabolism)
• Myositis, Inclusion Body (metabolism, pathology, physiopathology)
• Nestin (metabolism)
• PAX7 Transcription Factor (metabolism)
• Polymyositis (metabolism, pathology, physiopathology)
• RNA, Messenger (metabolism)
• Real-Time Polymerase Chain Reaction
• Regeneration
• Reverse Transcriptase Polymerase Chain Reaction
• Satellite Cells, Skeletal Muscle (metabolism, pathology)
• Signal Transduction
• Vimentin (metabolism)