Abstract |
Two species of DNA polymerase alpha free of primase activity were identified in extracts of Ehrlich mouse cells that had been infected with minute virus of mice. Primase-free forms of DNA polymerase alpha eluted with 150 and 180 mM NaCl during ion-exchange chromatography on DEAE-cellulose columns, exhibited sedimentation coefficients of 11 S and 8.2 S, respectively, and were inhibited by aphidicolin, N2-(p-n-butylphenyl)-9-(2-deoxy-beta-D-ribofuranosyl)guanine 5'-triphosphate, and 2-(p-n-butylanilino)-9-(2-deoxy-beta-D-ribofuranosyl)adenine 5'-triphosphate. The ratio of primase-free DNA polymerase alpha to the DNA polymerase alpha-primase complex increased from 1.5 to greater than 100 during the course of infection, and free primase was produced during the MVM replicative cycle.
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Authors | T F Ho, J S Gupta, E A Faust |
Journal | Biochemistry
(Biochemistry)
Vol. 28
Issue 11
Pg. 4622-8
(May 30 1989)
ISSN: 0006-2960 [Print] United States |
PMID | 2548583
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA, Viral
- Diterpenes
- Aphidicolin
- DNA Primase
- RNA Nucleotidyltransferases
- DNA Polymerase II
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Topics |
- Animals
- Aphidicolin
- Carcinoma, Ehrlich Tumor
(enzymology, microbiology)
- Cell Line
- Chromatography, Ion Exchange
(methods)
- DNA Polymerase II
(genetics, isolation & purification)
- DNA Primase
- DNA, Viral
(metabolism)
- Diterpenes
(pharmacology)
- Enzyme Induction
(drug effects)
- Mice
- Minute Virus of Mice
(genetics)
- Mutation
- Parvoviridae Infections
(enzymology, genetics)
- RNA Nucleotidyltransferases
(analysis)
- Virus Replication
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