Anti-inflammatory and anti-periductal fibrosis effects of an anthocyanin complex in Opisthorchis viverrini-infected hamsters.

Abstract

The pharmacological activities of herbal extracts can be enhanced by complex formation. In this study, we manipulated cyanidin and delphinidin-rich extracts to form an anthocyanin complex (AC) with turmeric and evaluated activity against inflammation and periductal fibrosis in Opisthorchis viverrini-infected hamsters. The AC was prepared from anthocyanins extracted from cobs of purple waxy corn (70%), petals of blue butterfly pea (20%) and turmeric extract (10%), resulting in an enhanced free-radical scavenging capacity. Oral administration of AC (175 and 700 mg/kg body weight) every day for 1 month to O. viverrini-infected hamsters resulted in reduced inflammatory cells and periductal fibrosis. Fourier transform infrared spectroscopy and partial least square discriminant analysis suggested nucleic acid changes in the O. viverrini-infected liver samples, which were partially prevented by the AC treatment. AC reduced 8-oxodG formation, an oxidative DNA damage marker, significantly decreased levels of nitrite in the plasma and alanine aminotransferase activity and increased the ferric reducing ability of plasma. AC also decreased the expression of oxidant-related genes (NF-κB and iNOS) and increased the expression of antioxidant-related genes (CAT, SOD, and GPx). Thus, AC increases free-radical scavenging capacity, decreases inflammation, suppresses oxidative/nitrative stress, and reduces liver injury and periductal fibrosis in O. viverrini-infected hamsters.

• Authors: Kitti Intuyod, Aroonsri Priprem, Wanwisa Limphirat, Lakhanawan Charoensuk, Porntip Pinlaor, Chawalit Pairojkul, Kamol Lertrat, Somchai Pinlaor
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 74 Pg. 206-15 (Dec 2014) ISSN: 1873-6351 [Electronic] England
• PMID: 25447758 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anthocyanins
• Anti-Inflammatory Agents
• Antioxidants
• Phenols
• Plant Extracts

Topics

• Animals
• Anthocyanins (chemistry, isolation & purification, pharmacology)
• Anti-Inflammatory Agents (pharmacology)
• Antioxidants (metabolism)
• Cricetinae
• Curcuma (chemistry)
• DNA Damage
• Gene Expression (drug effects)
• Liver Cirrhosis (pathology, prevention & control)
• Male
• Mesocricetus
• Opisthorchiasis (drug therapy, psychology)
• Opisthorchis
• Peas (chemistry)
• Phenols (pharmacology)
• Plant Extracts (pharmacology)
• Zea mays (chemistry)