Oral immunization with recombinant hepatitis E virus antigen displayed on the Lactococcus lactis surface enhances ORF2-specific mucosal and systemic immune responses in mice.

Abstract

Hepatitis E virus (HEV) as a recognized zoonotic pathogen has posed global burden on public health, which is exacerbated by lack of efficient vaccine. In this study, we constructed a recombinant (inaQ-ORF2 gene fusion) Lactococcus lactis (L. lactis) strain NZ3900 that expresses and displays the hepatitis E virus antigen ORF2 utilizing an ice uncleation protein-based anchor system. After oral vaccination of BALB/c mice, significantly higher levels of ORF2-specific mucosal IgA and serum IgG were detected and cellular immunity was also induced. These findings further support that L. lactis-based HEV antigen vaccines could be used for human and animal protection against infection.

• Authors: Shenyang Gao, Dandan Li, Ying Liu, Enhui Zha, Tiezhong Zhou, Xiqing Yue
• Journal: International immunopharmacology (Int Immunopharmacol) Vol. 24 Issue 1 Pg. 140-5 (Jan 2015) ISSN: 1878-1705 [Electronic] Netherlands
• PMID: 25445956 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier B.V. All rights reserved.

Chemical References

• Bacterial Outer Membrane Proteins
• Immunoglobulin A
• Immunoglobulin G
• ORF2 protein, Hepatitis E virus
• Recombinant Fusion Proteins
• Viral Hepatitis Vaccines
• Viral Proteins
• ice nucleation protein

Topics

• Administration, Oral
• Animals
• Bacterial Outer Membrane Proteins (genetics)
• Female
• Hepatitis E (immunology, prevention & control)
• Hepatitis E virus (immunology)
• Humans
• Immunity, Cellular
• Immunity, Mucosal
• Immunization
• Immunoglobulin A (blood)
• Immunoglobulin G (blood)
• Lactococcus lactis (genetics)
• Mice
• Mice, Inbred BALB C
• Recombinant Fusion Proteins (genetics)
• Viral Hepatitis Vaccines (administration & dosage, immunology)
• Viral Proteins (genetics, immunology)
• Zoonoses (immunology, prevention & control)