Abstract | AIM: Myocardial fibrosis is fundamental in the pathogenesis of heart failure. Late gadolinium enhancement (LGE) with cardiac magnetic resonance (CMR) imaging is commonly assumed to represent myocardial fibrosis; however, comparative human histological data are limited, especially in non-ischaemic cardiac disease. Diffuse interstitial myocardial fibrosis is increasingly recognized as central in the pathogenesis of cardiomyopathy and can be quantified using newer CMR techniques such as T1 mapping. We evaluated the relationship of CMR assessment of regional and diffuse fibrosis with human histology. METHODS AND RESULTS: Eleven patients on the waiting list for heart transplantation (43.5 ± 7.6 years, 64% male) and eight patients undergoing surgical myectomy for obstructive hypertrophic cardiomyopathy (57.1 ± 8.6 years, 63% male) were recruited and underwent CMR prior to cardiac transplantation or myectomy. Quantification of fibrosis in explanted hearts using digitally analysed Masson-trichrome-stained slides was validated against picrosirius red-stained slides analysed using Image J, with an excellent correlation (R = 0.95, P < 0.0001). Significant correlations were observed between LGE and histological fibrosis across a range of signal intensity thresholds in the explanted hearts (range: 2-10 standard deviations above reference myocardium), with maximal accuracy at a threshold of 6 SD (R = 0.91, P < 0.001). Assessment of interstitial myocardial fibrosis with post-contrast T1 times demonstrated a significant correlation on both segmental (R = -0.64, P = 0.002) and per-patient (R = -0.78, P = 0.003) analyses. CONCLUSION: CMR provides accurate, non-invasive assessment of regional myocardial fibrosis using LGE, while diffuse interstitial myocardial fibrosis is accurately assessed with post-contrast T1 mapping.
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Authors | Leah M Iles, Andris H Ellims, Huw Llewellyn, James L Hare, David M Kaye, Catriona A McLean, Andrew J Taylor |
Journal | European heart journal. Cardiovascular Imaging
(Eur Heart J Cardiovasc Imaging)
Vol. 16
Issue 1
Pg. 14-22
(Jan 2015)
ISSN: 2047-2412 [Electronic] England |
PMID | 25354866
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Validation Study)
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Copyright | Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2014. For permissions please email: [email protected]. |
Chemical References |
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Topics |
- Adult
- Biopsy, Needle
- Cardiac Surgical Procedures
(methods)
- Cardiomyopathy, Hypertrophic
(diagnosis, pathology, surgery)
- Disease Progression
- Endomyocardial Fibrosis
(diagnosis, pathology)
- Female
- Follow-Up Studies
- Gadolinium DTPA
- Heart Transplantation
(methods)
- Humans
- Immunohistochemistry
- Magnetic Resonance Imaging, Cine
(methods, standards)
- Male
- Middle Aged
- Prospective Studies
- Radiographic Image Enhancement
(methods)
- Risk Assessment
- Sampling Studies
- Sensitivity and Specificity
- Severity of Illness Index
- Waiting Lists
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