Ganoderma lucidum polysaccharide exerts anti-tumor activity via MAPK pathways in HL-60 acute leukemia cells.

Abstract

In this study, we investigated the anti-tumor activity both in vitro and in vivo of a polysaccharide obtained from Ganoderma lucidum on HL-60 acute myeloid leukemia cells, and focused on its targeting effect on mitogen-activated protein kinase (MAPK) pathways. It was found by the methods such as western blot and flow cytometry (FCM), that G. lucidum polysaccharide (GLP) blocked the extracellular signal-regulated kinase/MAPK signaling pathway, simultaneously activated p38 and JNK MAPK pathways, and therefore regulated their downstream genes and proteins, including p53, c-myc, c-fos, c-jun, Bcl-2, Bax, cleaved caspase-3 and cyclin D1. As a result, cycle arrest and apoptosis of HL-60 cells were induced. Therefore, GLP exerted anti-tumor activity via MAPK pathways in HL-60 acute leukemia cells.

• Authors: Guohua Yang, Lei Yang, Yun Zhuang, Xifeng Qian, Yunfeng Shen
• Journal: Journal of receptor and signal transduction research (J Recept Signal Transduct Res) Vol. 36 Issue 1 Pg. 6-13 ( 2016) ISSN: 1532-4281 [Electronic] England
• PMID: 25327706 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Polysaccharides
• JNK Mitogen-Activated Protein Kinases
• Mitogen-Activated Protein Kinases
• p38 Mitogen-Activated Protein Kinases

Topics

• Animals
• Antineoplastic Agents (isolation & purification, pharmacology)
• Apoptosis (drug effects)
• Blotting, Western
• Cell Cycle (drug effects)
• Cell Proliferation (drug effects)
• Cells, Cultured
• Female
• HL-60 Cells
• Humans
• JNK Mitogen-Activated Protein Kinases (metabolism)
• Leukemia (drug therapy, metabolism, pathology)
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Mitogen-Activated Protein Kinases (metabolism)
• Phosphorylation (drug effects)
• Polysaccharides (isolation & purification, pharmacology)
• Reishi (chemistry)
• Signal Transduction (drug effects)
• p38 Mitogen-Activated Protein Kinases (metabolism)