The purpose of the study was to determine multi-
vitamin deficiency effects on the inducibility of main
isoforms of
cytochrome P450 in the rat liver. The study was carried out on 4 groups of Wistar rats. Rats of the 1st and 3rd group received semi-synthetic diets containing adequate (100% of recommended
vitamin level) level of
vitamins, the 2nd and 4th--the semi-synthetic diet containing
vitamins in the amount of 20% from adequate level. The duration of the experiment was 4 weeks. During the last week
indole-3-carbinol (I-3-C) in dose of 20 mg/kg
body weight was added to the diet of the 3rd and 4th group of rats.
Vitamin E content in liver and blood serum declined by 59 and 34%, respectively in rats which were fed
vitamin-deficient diet (2nd group);
vitamin A level decreased by 5 times in the liver, but was not changed in blood serum. Multi-
vitamin deficiency in the diet led to the increase in the liver
ethoxyresorufin O-dealkylase (
EROD) activity of
CYP1A1, methoxyresorufin O-dealkylase (
MROD) activity of
CYP1A2 and testosteron 6beta-hydroxylase (6beta-TG) activity of
CYP3A by 11, 80 and 53%, respectively, and gene expression of
CYP1A1,
CYP1A2,
CYP3A and AhR by 8,5; 1,6; 2,4 and 3,6 fold. In rats fed diet with adequate levels of
vitamins (3rd group) I-3-C increased activity of
EROD and
MROD by 4,4 and 5,5 fold, and the expression of
CYP1A1,
CYP1A2 and AhR genes by 148; 3 and 3,5 fold compared to the parameters of the 1st group (without I-3-C). Multi-
vitamin deficiency increased I-3-C-related induction of
EROD activity and expression of
CYP1A1 and
CYP1A2 genes, but decreased I-3-C-related induction of the
MROD activity. Thus, 5-fold reducing of
vitamin content in rat diet lead to significant changes in activity and inducibility of
cytochrome P450 of CYP1A and 3A family, which play a key role in the detoxification and metabolism of drugs.