CD146 as a new marker for an increased chondroprogenitor cell sub-population in the later stages of osteoarthritis.

Abstract

Cartilage-derived mesenchymal stem cells (MSCs) have been isolated with different methods. In this study lateral and medial femoral condyles were respectively collected from patients with late-stage osteoarthritis during the total knee arthroplasty. After digestion of the cartilage tissues with type II collagenase and analysis by fluorescence-activated cell sorting (FACS) with CD146, a chondroprogenitor cell sub-population were isolated and purified. The expression of other MSC-associated markers in the CD146+ chondroprogenitors was analyzed by flow cytometry. Multi-lineage differentiation capacity of CD146+ chondroprogenitors was compared with that of unsorted chondrocytes and adipose-derived MSCs (ADMSCs). Higher percentage of CD146+ chondroprogenitors isolated from the medial femoral condyles was observed than that from the lateral. CD146+ chondroprogenitors expressed high levels of MSC-specific surface antigens, and showed higher chondrogenesis capacity than ADMSCs and unsorted chondrocytes in a 3D cell pellet culture model. Thus CD146 might be a new cell surface marker for cartilage progenitor cell population in the late-stage osteoarthritis.

• Authors: Xinlin Su, Wei Zuo, Zhihong Wu, Jun Chen, Nan Wu, Pei Ma, Zenan Xia, Chao Jiang, Zixing Ye, Sen Liu, Jiaqi Liu, Guangqian Zhou, Chao Wan, Guixing Qiu
• Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society (J Orthop Res) Vol. 33 Issue 1 Pg. 84-91 (Jan 2015) ISSN: 1554-527X [Electronic] United States
• PMID: 25266708 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.

Chemical References

• Biomarkers
• CD146 Antigen
• MCAM protein, human

Topics

• Adult
• Aged
• Arthroplasty, Replacement, Knee
• Biomarkers (metabolism)
• CD146 Antigen (metabolism)
• Case-Control Studies
• Cell Differentiation
• Cells, Cultured
• Chondrocytes (metabolism, pathology)
• Disease Progression
• Female
• Femur (metabolism, pathology, surgery)
• Humans
• Immunophenotyping
• Knee Joint (metabolism, pathology, surgery)
• Male
• Middle Aged
• Osteoarthritis, Knee (metabolism, pathology)
• Severity of Illness Index
• Stem Cells (metabolism, pathology)