Preparation and in vivo efficient anti-infection property of GTR/GBR implant made by metronidazole loaded electrospun polycaprolactone nanofiber membrane.

Abstract

Infection is the major reason of GTR/GBR membrane failure in clinical application. In this work, we developed GTR/GBR nanofiber membranes with localized drug delivery function to prevent infection. Metronidazole (MNA), an antibiotic, was successfully incorporated into electrospun polycaprolactone (PCL) nanofibers at different concentrations (0, 1, 5, 10, 20, 30, and 40 wt% polymer). To obtain the optimum anti-infection membrane, we systematically investigated the physical-chemical and mechanical properties of the nanofiber membranes with different drug contents. The interaction between PCL and MNA was identified by molecular dynamics simulation. MNA released in a controlled, sustained manner over 2 weeks and the antibacterial activity of the released MNA remained. The incorporation of MNA improved the hydrophilicity and in vitro biodegradation rate of PCL nanofibers. The nanofiber membranes allowed cells to adhere to and proliferate on them and showed excellent barrier function. The membrane loaded with 30% MNA had the best comprehensive properties. Analysis of subcutaneous implants demonstrated that MNA-loaded nanofibers evoked a less severe inflammatory response than pure PCL nanofibers. These results demonstrate the potential of MNA-loaded nanofiber membranes as GTR/GBR membrane with antibacterial and anti-inflammatory function for extensive biomedical applications.

• Authors: Jiajia Xue, Min He, Yuzhao Niu, Hao Liu, Aileen Crawford, Phil Coates, Dafu Chen, Rui Shi, Liqun Zhang
• Journal: International journal of pharmaceutics (Int J Pharm) Vol. 475 Issue 1-2 Pg. 566-77 (Nov 20 2014) ISSN: 1873-3476 [Electronic] Netherlands
• PMID: 25240438 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier B.V. All rights reserved.

Chemical References

• Anti-Infective Agents
• Biocompatible Materials
• Metronidazole
• Caprolactam
• capron

Topics

• Animals
• Anti-Infective Agents (administration & dosage, chemistry)
• Biocompatible Materials (chemistry)
• Bone Regeneration
• Caprolactam (chemistry)
• Cell Proliferation
• Dose-Response Relationship, Drug
• Drug Delivery Systems
• Drug Liberation
• Fibroblasts (drug effects)
• Guided Tissue Regeneration (methods)
• Male
• Metronidazole (administration & dosage, chemistry)
• Microbiological Techniques
• Nanofibers (chemistry)
• Prostheses and Implants
• Rabbits