Abstract |
The goal of the present study was to investigate the impact of tetrabromobisphenol A (TBBPA) on human choriocarcinoma-derived placental JEG-3 cells in vitro. We determined the effect of this compound on estradiol secretion, aromatase protein expression and activity in vitro in the JEG-3 cell line. We assessed the ability of TBBPA to increase intracellular levels of cAMP as well as its effect on cell viability and proliferation. Our results indicated that TBBPA, at a wide range of concentrations (1×10(-8)-5×10(-5)M), significantly induced estradiol secretion by JEG-3 cells compared to that of controls after 24, 48 or 72 h of exposure. This effect was accompanied by an increase in the aromatase protein expression in JEG-3 cells treated with 100 nM and 10 μM of TBBPA for 24 h. Additionally, in our study, we confirmed that TBBPA-induced changes in aromatase protein expression were associated w ith the up-regulation of aromatase activity and cAMP levels. No tested doses of TBBPA inhibited JEG-3 cell proliferation, except for the highest dose of 100 μM, which had a toxic effect on cell viability at all time points. The present study clearly indicates that TBBPA alters JEG-3 cells estrogen synthesis due to its action on CYP19 protein expression and thus this compound may interfere with normal placental development during early pregnancy.
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Authors | Ewelina Honkisz, Anna K Wójtowicz |
Journal | Toxicology in vitro : an international journal published in association with BIBRA
(Toxicol In Vitro)
Vol. 29
Issue 1
Pg. 44-50
(Feb 2015)
ISSN: 1879-3177 [Electronic] England |
PMID | 25223798
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Elsevier Ltd. All rights reserved. |
Chemical References |
- Endocrine Disruptors
- Flame Retardants
- Polybrominated Biphenyls
- Estradiol
- Cyclic AMP
- Aromatase
- CYP19A1 protein, human
- tetrabromobisphenol A
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Topics |
- Aromatase
(metabolism)
- Blotting, Western
- Cell Proliferation
(drug effects)
- Choriocarcinoma
(chemistry, enzymology, metabolism)
- Cyclic AMP
(analysis)
- Dose-Response Relationship, Drug
- Endocrine Disruptors
(toxicity)
- Estradiol
(biosynthesis)
- Female
- Flame Retardants
(toxicity)
- Humans
- Polybrominated Biphenyls
(toxicity)
- Tumor Cells, Cultured
- Uterine Neoplasms
(chemistry, enzymology, metabolism)
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