Abstract | BACKGROUND/AIM: The present study aimed to investigate anticancer properties of equol and demonstrate its underlying mechanisms of action in human cervical cancer HeLa cells. MATERIALS AND METHODS: Inhibition of cell viability was examined by 3-(4,5-dimethylthiazoly-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Apoptosis was evaluated by observation of apoptotic cell morphology, and an increase of annexin-V(+) cells. Western blotting was used to examine apoptosis-related proteins. Flow cytometry was used to measure mitochondrial membrane potential ( MMP) and reactive oxygen species (ROS). RESULTS: CONCLUSION:
Equol is a potential anticancer agent against HeLa, with possible mechanisms involved in ROS generation and mitochondrial membrane alteration.
|
Authors | Eun Young Kim, Jin Young Shin, Young-Ja Park, An Keun Kim |
Journal | Anticancer research
(Anticancer Res)
Vol. 34
Issue 9
Pg. 4985-92
(Sep 2014)
ISSN: 1791-7530 [Electronic] Greece |
PMID | 25202081
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright© 2014 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved. |
Chemical References |
- Proto-Oncogene Proteins c-bcl-2
- Reactive Oxygen Species
- Equol
- Caspases
|
Topics |
- Apoptosis
(drug effects)
- Caspases
(metabolism)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Dose-Response Relationship, Drug
- Equol
(pharmacology)
- Female
- HeLa Cells
- Humans
- Membrane Potential, Mitochondrial
(drug effects)
- Mitochondria
(drug effects, metabolism)
- Proto-Oncogene Proteins c-bcl-2
(metabolism)
- Reactive Oxygen Species
(metabolism)
- Signal Transduction
(drug effects)
- Uterine Cervical Neoplasms
(metabolism, pathology)
|