Evaluation of protective immune response in mice by vaccination the recombinant adenovirus for expressing Schistosoma japonicum inhibitor apoptosis protein.

Abstract

Schistosomiasis is a worldwide parasitic disease, and while it can be successfully treated with chemotherapy, this does not prevent reinfection with the parasite. Adenovirus vectors have been widely used for vaccine delivery, and a vaccination approach has the potential to prevent infection with Schistosoma. Here, we developed a recombinant adenoviral vector that expresses Schistosoma japonicum inhibitor apoptosis protein (Ad-SjIAP) and assessed its immunoprotective functions against schistosomiasis in mice. Murine immune responses following vaccination were investigated using enzyme-linked immunosorbent assays (ELISA), lymphocyte proliferation, and cytokine assays. The protective immunity in mice was evaluated by challenging with S. japonicum cercariae. Our results indicated that immunization with the Ad-SjIAP in mice induced a strong serum IgG response against IAP including IgG1, IgG2a, and IgG2b. In addition, lymphocyte proliferation experiments showed that mice treated with Ad-SjIAP significantly increased the lymphocyte response upon stimulation with recombinant Schistosoma japonicum inhibitor apoptosis protein (rSjIAP). Moreover, cytokine assays indicated that vaccination of Ad-SjIAP significantly increased the production of interferon (IFN)-γ and IL-2 as compared to the corresponding control group. Furthermore, following the challenge with S. japonicum cercariae, the vaccine conferred moderate protection, with an average rate of 37.95% for worm reduction and 31.7% for egg reduction. Taken together, our preliminarily results suggested that schistosoma IAP may be a potential vaccine against S. japonicum and that adenoviral vectors may serve as an alternative delivery vehicle for schistosome vaccine development.

• Authors: Chao Hu, Lihui Zhu, Rong Luo, Jinwei Dao, Jiangping Zhao, Yaojun Shi, Hao Li, Ke Lu, Xingang Feng, Jiaojiao Lin, Jinming Liu, Guofeng Cheng
• Journal: Parasitology research (Parasitol Res) Vol. 113 Issue 11 Pg. 4261-9 (Nov 2014) ISSN: 1432-1955 [Electronic] Germany
• PMID: 25185668 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Helminth
• Helminth Proteins
• Immunoglobulin G
• Inhibitor of Apoptosis Proteins
• Interleukin-2
• Vaccines, Synthetic
• Interferon-gamma

Topics

• Adenoviridae
• Animals
• Antibodies, Helminth (blood)
• Enzyme-Linked Immunosorbent Assay
• Helminth Proteins (immunology)
• Immunoglobulin G (blood)
• Inhibitor of Apoptosis Proteins (immunology)
• Interferon-gamma (immunology)
• Interleukin-2 (immunology)
• Lymphocyte Activation (immunology)
• Mice, Inbred BALB C
• Parasite Egg Count
• Schistosoma japonicum
• Schistosomiasis japonica (immunology)
• Spleen (cytology, immunology)
• Vaccines, Synthetic (immunology)