Abstract | OBJECTIVE: METHODS: RESULTS:
Type I collagen expressions were detected in both the lung cancer tissues and the cell lines. In the 3D culture system, the growth of the cancer cell lines was obviously suppressed by shRNA-mediated type I collagen knockdown evidenced by lowered cell growth rate and colony formation ability. Stimulation with fibroblast culture supernatant resulted in enhanced expressions of type I collagen in the cancer cell lines. CONCLUSION: Autocrine of type I collagen I is required for maintaining lung cancer cell growth in 3D cultures, and its expression is regulated by fibroblasts. These findings provide new insights into the role of type I collagen I in the tumor microenvironment and point a new direction for targeted therapy of tumors.
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Authors | Jiangchao Li, Xiaomei Li, Tian Lan, Cuiling Qi, Xiaodong He, Hong Yang, Yan Li, Lijing Wang, Xinyuan Guan |
Journal | Nan fang yi ke da xue xue bao = Journal of Southern Medical University
(Nan Fang Yi Ke Da Xue Xue Bao)
Vol. 34
Issue 8
Pg. 1129-34
(Jul 2014)
ISSN: 1673-4254 [Print] China |
PMID | 25176080
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Collagen Type I
- RNA, Messenger
- RNA, Small Interfering
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Topics |
- Cell Cycle
- Cell Line, Tumor
- Cell Proliferation
- Collagen Type I
(metabolism)
- Fibroblasts
- Humans
- Immunohistochemistry
- Lung Neoplasms
(metabolism)
- RNA, Messenger
- RNA, Small Interfering
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