Three-dimensional tracking of Rab5- and Rab7-associated infection process of influenza virus.

Abstract

Three-dimensional (3D) single-particle tracking (SPT) techniques have been widely reported. However, the 3D SPT technique remains poorly used for solving actual biological problems. In this work, a quantum dots (QDs)-based single-particle tracking technique is utilized to explore the Rab5- and Rab7-associated infection behaviors of influenza virus in three dimensions with a set of easily-attained equipment by the fast and accurate centroid method for 3D SPT. The experimental results indicate that Rab5 protein takes part in the virus infection process from the cell periphery to the perinuclear region, while Rab7 protein is mainly involved in the intermittent and confined movements of the virus in the perinuclear region. Evidently, the transition process of the virus-containing vesicles from early to late endosomes might occur during the intermittent movement in the perinuclear region. These findings reveal distinct dynamic behaviors of Rab5- and Rab7-positive endosomes in the course of the intracellular transport of viruses. This work is helpful in understanding the intracellular transport of cargoes.

• Authors: Shu-Lin Liu, Qiu-Mei Wu, Li-Juan Zhang, Zhi-Gang Wang, En-Ze Sun, Zhi-Ling Zhang, Dai-Wen Pang
• Journal: Small (Weinheim an der Bergstrasse, Germany) (Small) Vol. 10 Issue 22 Pg. 4746-53 (Nov 2014) ISSN: 1613-6829 [Electronic] Germany
• PMID: 24976105 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• rab7 GTP-Binding Proteins
• rab7 GTP-binding proteins, human
• rab GTP-Binding Proteins
• rab5 GTP-Binding Proteins

Topics

• Animals
• Dogs
• Humans
• Influenza, Human (prevention & control)
• Madin Darby Canine Kidney Cells
• rab GTP-Binding Proteins (physiology)
• rab5 GTP-Binding Proteins (physiology)
• rab7 GTP-Binding Proteins