Abstract | PURPOSE: MATERIALS AND METHODS: Mouse NAFLD models with varying severity were created by feeding high-fat, high- cholesterol (HFHC) diets to ob/ob mice for 3, 6, or 12 weeks. SPIO-MRI was performed on a 4.7-T animal scanner in the mouse NAFLD models, in wildtype control mouse, and in the NAFLD mice ( NAFLD treatment group) that received 6 weeks of pioglitazone treatment. The relative signal loss (RSL) of the liver was measured in each animal to represent the magnitude of SPIO-induced signal loss of the liver. Liver samples were analyzed for steatosis, inflammation, fibrosis, and the number of SPIO particles and KCs. RESULTS: RSL values of the NAFLD mice (range of RSL value, 26.3%-53.8%) seen on SPIO-MRI were significantly lower than those of the control mice (67.7%-74.8%, P ≤ 0.008) and decreased in proportion to the duration of their HFHC diet (mean ± SD, 53.7% ± 10.9, 44.7% ± 8.2, and 26.3% ± 12.6, after 3-, 6-, and 12-week HFHC diet, respectively, on 20-minute delayed images). For the NAFLD treatment group, the RSL values increased after 6 weeks of pioglitazone treatment, compared with the values before treatment (P ≤ 0.039). The RSL values had significant independent correlation with both hepatic steatosis (P = 0.007) and inflammation (P = 0.023). CONCLUSION: KC phagocytic dysfunction is aggravated in the progression of NAFLD and may be reversible with therapeutic intervention. SPIO-MRI may be useful for classifying the severity of NAFLD and monitoring the treatment response of NAFLD.
|
Authors | Hyunhee Cheong, Seung Soo Lee, Jin Seong Lee, Jihoon Kim, Seong Who Kim, Woo Je Lee |
Journal | Journal of magnetic resonance imaging : JMRI
(J Magn Reson Imaging)
Vol. 41
Issue 5
Pg. 1218-27
(May 2015)
ISSN: 1522-2586 [Electronic] United States |
PMID | 24916329
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | © 2014 Wiley Periodicals, Inc. |
Chemical References |
- Contrast Media
- Dextrans
- Magnetite Nanoparticles
- ferumoxtran-10
|
Topics |
- Animals
- Contrast Media
- Dextrans
- Image Enhancement
(methods)
- Image Interpretation, Computer-Assisted
(methods)
- Kupffer Cells
(pathology)
- Magnetic Resonance Imaging
(methods)
- Magnetite Nanoparticles
- Male
- Mice
- Mice, Inbred C57BL
- Non-alcoholic Fatty Liver Disease
(pathology, physiopathology)
- Phagocytosis
- Reproducibility of Results
- Sensitivity and Specificity
|