Mixed T helper cell signatures in chronic rhinosinusitis with and without polyps.

Abstract

UNLABELLED:

In chronic rhinosinusitis (CRS) different phenotypes have been reported based on cytokine profile and inflammatory cell patterns. The aim of this study was to characterize the intracytoplasmatic cytokines of T cells infiltrating the inflamed sinonasal mucosa.

METHODS:

Infiltrated T cells and tissue homogenates from sinonasal mucosal samples of 7 healthy subjects, 9 patients with CRS without nasal polyp (CRSsNP), 15 with CRS with nasal polyps (CRSwNP) and 5 cystic fibrosis patients (CF-NP) were analyzed for cytokine expression using flow cytometry and multiplex analysis respectively. Intracytoplasmic cytokinesin T cells were analyzed after stimulation of nasal polyps with Staphylococcus aureus enterotoxin B for 24 hours.

RESULTS:

The number of T cells per total living cells was significantly higher in patients with CRSwNP vs. CRSsNP and controls. 85% of the CD4(+) T cells showed to be memory T cells. The effector T cells present in all tissues have a predominant Th1 phenotype. Only in CRSwNP, a significant fraction of T cells produced the Th2 cytokines IL-4 and IL-5, while nasal polyps from CF patients were characterized by a higher CD4/CD8 T cell ratio and an increased number of Th17 cells. 24 h stimulation with SEB resulted in a significant induction of CD4(+) T cells producing IL-10 (Tr1 cells).

CONCLUSION:

T cell cytokine patterns in healthy and inflamed sinonasal mucosa revealed that Th2 cells (IL-4 and IL-5 producing cells) are significantly increased in CRSwNP mucosal inflammation. Exposure to SEB stimulates Tr1 cells that may contribute to the Th2 bias in CRSwNP.

• Authors: Lara Derycke, Stefanie Eyerich, Koen Van Crombruggen, Claudina Pérez-Novo, Gabriele Holtappels, Natalie Deruyck, Philippe Gevaert, Claus Bachert
• Journal: PloS one (PLoS One) Vol. 9 Issue 6 Pg. e97581 ( 2014) ISSN: 1932-6203 [Electronic] United States
• PMID: 24911279 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines
• Enterotoxins
• enterotoxin B, staphylococcal

Topics

• Adult
• CD4-Positive T-Lymphocytes (cytology, drug effects)
• CD8-Positive T-Lymphocytes (cytology, drug effects)
• Case-Control Studies
• Chronic Disease
• Cytokines (metabolism)
• Enterotoxins (toxicity)
• Female
• Humans
• Male
• Middle Aged
• Nasal Polyps (complications)
• Sinusitis (complications, immunology)
• T-Lymphocytes, Helper-Inducer (cytology, drug effects)
• Young Adult