Magnolol reduced TNF-α-induced vascular cell adhesion molecule-1 expression in endothelial cells via JNK/p38 and NF-κB signaling pathways.

Abstract

Expression of cell adhesion molecules by the endothelium and the attachment of leukocytes to these cells play major roles in inflammation and cardiovascular disorders. Magnolol, a major active component of Magnolia officinalis, has antioxidative and anti-inflammatory properties. In the present study, the effects of magnolol on the expression of vascular cell adhesion molecule-1 (VCAM-1) in human aortic endothelial cells (HAECs) and the related mechanisms were investigated. TNF-α induced VCAM-1 protein expression and mRNA stability were significantly decreased in HAECs pre-treated with magnolol. Magnolol significantly reduced the phosphorylation of ERK, JNK, and p38 in TNF-α-treated HAECs. The decrease in VCAM-1 expression in response to TNF-α treatment was affected by JNK and p38 inhibitors, not by an ERK inhibitor. Magnolol also attenuates NF-κB activation and the translocation of HuR (an RNA binding protein) in TNF-α-stimulated HAECs. The VCAM-1 expression was weaker in the aortas of TNF-α-treated apo-E deficient mice with magnolol treatment. These data demonstrate that magnolol inhibits TNF-α-induced JNK/p38 phosphorylation, HuR translocation, NF-κB activation, and thereby suppresses VCAM-1 expression resulting in reduced leukocyte adhesion. Taken together, these results suggest that magnolol has an anti-inflammatory property and may play an important role in the prevention of atherosclerosis and inflammatory responses.

• Authors: Chan-Jung Liang, Chiang-Wen Lee, Hsin-Ching Sung, Yung-Hsiang Chen, Shu-Huei Wang, Pei-Jhen Wu, Yao-Chang Chiang, Jaw-Shiun Tsai, Chau-Chung Wu, Chi-Yuan Li, Yuh-Lien Chen
• Journal: The American journal of Chinese medicine (Am J Chin Med) Vol. 42 Issue 3 Pg. 619-37 ( 2014) ISSN: 1793-6853 [Electronic] Singapore
• PMID: 24871655 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Antioxidants
• Apolipoproteins E
• Biphenyl Compounds
• Lignans
• NF-kappa B
• Tumor Necrosis Factor-alpha
• Vascular Cell Adhesion Molecule-1
• magnolol

Topics

• Animals
• Anti-Inflammatory Agents
• Antioxidants
• Aorta (cytology, drug effects)
• Apolipoproteins E (deficiency)
• Atherosclerosis (prevention & control)
• Biphenyl Compounds (pharmacology)
• Cells, Cultured
• Endothelial Cells (metabolism)
• Gene Expression (drug effects, genetics)
• Humans
• Lignans (pharmacology)
• MAP Kinase Signaling System (drug effects, genetics, physiology)
• Mice
• NF-kappa B (genetics, physiology)
• Phosphorylation (drug effects, genetics)
• Phytotherapy
• Signal Transduction (drug effects, genetics, physiology)
• Tumor Necrosis Factor-alpha (adverse effects)
• Vascular Cell Adhesion Molecule-1 (genetics, metabolism)