Soluble CD40 ligand-activated B cells from patients with chronic hepatitis B virus infection as antigen presenting cells to induce hepatitis B virus specific cytotoxic T lymphocytes.

Abstract

Chronic hepatitis B virus (HBV) infection is the result of an inadequate antiviral immune response to the virus. In this study, we aimed to investigate whether the soluble CD40 ligand-activated B (CD40-B) cells could present antigen and induce specific cytotoxic T lymphocytes (CTLs) in patients with chronic HBV infection. We observed that after activated by sCD40L, the expression of CD80, CD86, major histocompatibility complex (MHC) I and II molecules on the CD40-B cells was significantly increased. Cytometry and fluorescence microscopy showed that more than 41.34% CD40-B cells were loaded by the HBcAg peptide. Furthermore, after been activated and HBcAg18-27 antigen peptide pulsed, B cells obtained from patients with chronic HBV infection could induce HBcAg18-27 specific CTLs in vitro. Taken together, our results show that B cells from patients with chronic HBV infection can be activated by sCD40L and may function as antigen presenting cells and induce HBV-specific CTLs.

• Authors: Yong Liu, Rui Huang, Yali Xiong, Qi Zhao, Guangmei Chen, Juan Xia, Chao Wu
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 450 Issue 1 Pg. 61-6 (Jul 18 2014) ISSN: 1090-2104 [Electronic] United States
• PMID: 24866241 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.

Chemical References

• CD40 Antigens
• Hepatitis B Core Antigens

Topics

• Antigen-Presenting Cells (immunology, virology)
• B-Lymphocytes (immunology, virology)
• CD40 Antigens (immunology)
• Cells, Cultured
• Hepatitis B Core Antigens (immunology)
• Hepatitis B virus (immunology)
• Hepatitis B, Chronic (immunology, virology)
• Humans
• Solubility
• T-Lymphocytes, Cytotoxic (immunology, virology)