Impact of GNB3-C825T, ADRB3-Trp64Arg, UCP2-3'UTR 45 bp del/ins, and PPARγ-Pro12Ala polymorphisms on Bofutsushosan response in obese subjects: a randomized, double-blind, placebo-controlled trial.

Abstract

Obesity is known to be influenced by a number of genes, including the β3 subunit of G protein (GNB3), β3-adrenergic receptor (ADRB3), uncoupling protein 2 (UCP2), and peroxisome proliferator activated receptor gamma (PPARγ). The single nucleotide polymorphisms (SNPs) of the above genes, such as GNB3-C825T, ADRB3-Trp64Arg, UCP2-3'UTR 45 bp del/ins, and PPARγ-Pro12Ala, are associated with obesity and body mass index. The present study evaluates the impact of Bofutsushosan, a traditional Eastern Asian herbal medicine with known anti-obesity properties, on obese subjects according to the presence of the above-mentioned SNPs. Upon randomization, the volunteers were allocated to receive Bofutsushosan (n=55) or placebo (n=56) treatments for 8 weeks. Following the treatment schedule, significant reductions in total cholesterol and significant improvement in the Korean version of obesity-related quality of life scale were seen in the Bofutsushosan-treated group, but not in placebo. Bofutsushosan exerted significant anti-obesity effects on a number of parameters in the carriers of the GNB3-825T allele, but only on waist circumference in the GNB3-C/C homozygote. Significant anti-obesity impact of Bofutsushosan was also seen on a number of obesity-indices in both ADRB3-Arg64 carriers and ADRB3-Trp64 homozygotes, as well as in UCP2-D/D carriers, but not in UCP2-D/I+I/I variants. The effect of Bofutsushosan was more pronounced in PPARγ-Pro/Pro genotype compared to PPARγ-Pro/Ala variants. Thus, the results revealed differential responses of the subjects to the anti-obesity effects of Bofutsushosan treatment according to the polymorphism of the vital obesity-related genes. Our study provides new insight into individualized clinical applications of Bofutsushosan for obesity.

• Authors: Junghyun Park, Shambhunath Bose, Sun-Woo Hong, Dong-Ki Lee, Jae-Wook Yoo, Chi-Yeon Lim, Myeongjong Lee, Hojun Kim
• Journal: Journal of medicinal food (J Med Food) Vol. 17 Issue 5 Pg. 558-70 (May 2014) ISSN: 1557-7600 [Electronic] United States
• PMID: 24827746 (Publication Type: Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)

Chemical References

• 3' Untranslated Regions
• ADRB3 protein, human
• Anti-Obesity Agents
• Drugs, Chinese Herbal
• G-protein beta3 subunit
• Ion Channels
• Mitochondrial Proteins
• PPAR gamma
• Placebos
• Receptors, Adrenergic, beta-3
• UCP2 protein, human
• Uncoupling Protein 2
• bofu-tsusho-san
• Heterotrimeric GTP-Binding Proteins

Topics

• 3' Untranslated Regions
• Adult
• Anti-Obesity Agents (therapeutic use)
• Body Mass Index
• Double-Blind Method
• Drugs, Chinese Herbal (therapeutic use)
• Genotype
• Heterotrimeric GTP-Binding Proteins (genetics)
• Humans
• Ion Channels (genetics)
• Middle Aged
• Mitochondrial Proteins (genetics)
• Mutation
• Obesity (drug therapy, genetics)
• PPAR gamma (genetics)
• Placebos
• Polymorphism, Single Nucleotide
• Receptors, Adrenergic, beta-3 (genetics)
• Republic of Korea
• Uncoupling Protein 2
• Waist Circumference