Antibody-independent, complement-mediated enhancement of HIV-1 infection by mannosidase I and II inhibitors.

Abstract

Human immunodeficiency virus type 1 (HIV-1) infectivity and cytopathic effect require proper maturation of the viral envelope glycoprotein carbohydrate moieties. We have found that fresh human serum enhances the infectivity of HIV-1 in MT-2 cell infection assays when virus is synthesized in the presence of the mannosidase I inhibitor, 1-deoxymannojirimycin, or the mannosidase II inhibitor, swainsonine, but has no enhancing effect on virus synthesized in the presence of the glucosidase I inhibitors, castanospermine and 1-deoxynojirimycin, or the glucosidase II inhibitor, bromoconduritol. Enhanced infections were characterized by cytopathic effect, antigen synthesis and reverse transcriptase release, all which occurred sooner than in control-infected cultures. This enhancement of infection was also observed in C1q-deficient serum but was not observed in serum that was heat-inactivated or depleted of complement components C3 or factor B, thus suggesting a requirement for the alternate pathway of complement.

• Authors: D C Montefiori, W E Robinson Jr, W M Mitchell
• Journal: Antiviral research (Antiviral Res) Vol. 11 Issue 3 Pg. 137-46 (Apr 1989) ISSN: 0166-3542 [Print] Netherlands
• PMID: 2472115 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Alkaloids
• Complement C3
• 1-Deoxynojirimycin
• Complement System Proteins
• RNA-Directed DNA Polymerase
• Glucosidases
• Mannosidases
• mannosyl-oligosaccharide 1,2-alpha-mannosidase
• mannosyl-oligosaccharide 1,3 - 1,6-alpha-mannosidase
• Complement Factor B
• Glucosamine
• Swainsonine

Topics

• 1-Deoxynojirimycin
• Alkaloids (pharmacology)
• Cells, Cultured
• Complement C3 (immunology)
• Complement Factor B (immunology)
• Complement System Proteins (immunology)
• Cytopathogenic Effect, Viral (drug effects)
• Fluorescent Antibody Technique
• Glucosamine (analogs & derivatives, pharmacology)
• Glucosidases (antagonists & inhibitors)
• Glycosylation
• HIV-1 (drug effects, immunology, isolation & purification, pathogenicity)
• Humans
• Mannosidases (antagonists & inhibitors)
• RNA-Directed DNA Polymerase (metabolism)
• Swainsonine