Abstract | BACKGROUND: Evidence suggests that many types of cancers are composed of different cell types, including cancer stem cells (CSCs). We have previously shown that the chemotherapeutic agent oxaliplatin induced epithelial-mesenchymal transition, which is thought to be an important mechanism for generating CSCs. In the present study, we investigate whether oxaliplatin-treated cancer tissues possess characteristics of CSCs, and explore oxaliplatin resistance in these tissues. METHODS:
Hepatocellular carcinoma cells (MHCC97H cells) were subcutaneously injected into mice to form tumors, and the mice were intravenously treated with either oxaliplatin or glucose. Five weeks later, the tumors were orthotopically xenografted into livers of other mice, and these mice were treated with either oxaliplatin or glucose. Metastatic potential, sensitivity to oxaliplatin, and expression of CSC-related markers in the xenografted tumor tissues were evaluated. DNA microarrays were used to measure changes in gene expression as a result of oxaliplatin treatment. Additionally, an oxaliplatin-resistant cell line (MHCC97H-OXA) was established to assess insulin-like growth factor 1 secretion, cell invasion, cell colony formation, oxaliplatin sensitivity, and expression of CSC-related markers. The effects of an insulin-like growth factor 1 receptor inhibitor were also assessed. RESULTS: CONCLUSION:
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Authors | Yang Bu, Qing-An Jia, Zheng-Gang Ren, Ju-Bo Zhang, Xue-Mei Jiang, Lei Liang, Tong-Chun Xue, Quan-Bao Zhang, Yan-Hong Wang, Lan Zhang, Xiao-Ying Xie, Zhao-You Tang |
Journal | PloS one
(PLoS One)
Vol. 9
Issue 3
Pg. e89686
( 2014)
ISSN: 1932-6203 [Electronic] United States |
PMID | 24632571
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Organoplatinum Compounds
- Oxaliplatin
- Insulin-Like Growth Factor I
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Topics |
- Animals
- Antineoplastic Agents
(therapeutic use)
- Carcinoma, Hepatocellular
(drug therapy, metabolism)
- Cell Line, Tumor
- Drug Resistance, Neoplasm
- Epithelial-Mesenchymal Transition
(drug effects)
- Insulin-Like Growth Factor I
(metabolism)
- Liver Neoplasms
(drug therapy, metabolism)
- Mice
- Mice, Nude
- Neoplastic Stem Cells
(drug effects)
- Organoplatinum Compounds
(therapeutic use)
- Oxaliplatin
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