Comparison of HMB-45 monoclonal antibody and S-100 protein in the immunohistochemical diagnosis of melanoma.

Abstract

The value of HMB-45 mouse monoclonal antibody in the immunohistochemical diagnosis of melanoma was compared with that of two antibodies to S-100 protein. Tissue from 32 (91.4%) of the 35 melanomas studied reacted with HMB-45, however, none of the 98 nonmelanoma tumors stained with this antibody. In contrast, 31 (88.5%) melanomas and 24 (24.4%) of the nonmelanoma neoplasms expressed S-100 protein. These results indicated that HMB-45 monoclonal antibody has a higher specificity for melanoma cells than did S-100 protein and that, because of its reactivity in routinely processed tissues, it may be helpful in surgical pathology.

• Authors: N G Ordóñez, X L Ji, R C Hickey
• Journal: American journal of clinical pathology (Am J Clin Pathol) Vol. 90 Issue 4 Pg. 385-90 (Oct 1988) ISSN: 0002-9173 [Print] England
• PMID: 2459952 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Antigens, Neoplasm
• Indicators and Reagents
• Melanoma-Specific Antigens
• Neoplasm Proteins
• S100 Proteins

Topics

• Antibodies, Monoclonal
• Antigen-Antibody Reactions
• Antigens, Neoplasm
• Humans
• Immunohistochemistry (methods)
• Indicators and Reagents
• Melanoma (analysis, pathology)
• Melanoma-Specific Antigens
• Neoplasm Metastasis
• Neoplasm Proteins (analysis)
• S100 Proteins (analysis, immunology)
• Staining and Labeling (methods)