Abstract | OBJECTIVES/HYPOTHESIS: STUDY DESIGN: Retrospective review of clinical pathologic specimens and in vivo animal study. METHODS: RESULTS: All tumor samples demonstrated significant FR-β staining and negligible FR-α staining. FR-β(+) cells found in tumors coexpressed CD68 and had increased expression of CD206 and TGF-β characteristic of tumor-associated macrophages. In the xenograft models, tumors showed strong in vivo fluorescence after folate- FITC injection in contrast to surrounding normal tissues. Histologic examination of the xenograft tissue similarly showed folate- FITC uptake in areas of inflammatory cellular infiltrate. CONCLUSIONS: Although HNSCC tumor cells do not express FR, HNSCC tumors contain a significant population of FR-β-expressing macrophages. Folate conjugated fluorescent dye is able to specifically target and label tumor xenografts to permit macroscopic fluorescence imaging due to FR-β expression on the infiltrating inflammatory cells.
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Authors | Joel Y Sun, Jiayin Shen, Joel Thibodeaux, Gang Huang, Yiguang Wang, Jinming Gao, Philip S Low, Dimiter S Dimitrov, Baran D Sumer |
Journal | The Laryngoscope
(Laryngoscope)
Vol. 124
Issue 8
Pg. E312-9
(Aug 2014)
ISSN: 1531-4995 [Electronic] United States |
PMID | 24448885
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2014 The American Laryngological, Rhinological and Otological Society, Inc. |
Chemical References |
- Folate Receptor 1
- Folate Receptor 2
- Neoplasm Proteins
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Animals
- Carcinoma, Squamous Cell
(chemistry, pathology)
- Female
- Folate Receptor 1
- Folate Receptor 2
(analysis)
- Head and Neck Neoplasms
(chemistry, pathology)
- Humans
- Mice
- Middle Aged
- Neoplasm Proteins
(analysis)
- Optical Imaging
- Retrospective Studies
- Squamous Cell Carcinoma of Head and Neck
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